USDA, ARS, FMOD, BA, Contracting Section, Bldg. 003, Rm. 329, 10300 Baltimore Ave., Beltsville, Maryland 20705-2350

66 -- BENCH TOP CENTRIFUGAL CHEMISTRY ANALYZER SOL 09-3K47-97 DUE 032897 POC Keina Burrier, Contract Specialist, (301) 504-5888 This is a combined synopsis/solicitation for commercial item prepared in accordance with FAR Subpart 12.6 as supplemented with additional information included in this notice. The announcement constitutes the only solicitation; proposals are being requested and a written solicitation will not be issued. The solicitation number 09-3K47-97, is issued as a Request for Proposals (RFP), and incorporates provisions and clauses that in effect through Federal Acquisition Circular 90-42. The standard industrial classification code is 3826, with a small business standard of 500 employees. The acquisition will be procured on an unrestricted basis. The Government intends to negotiate non-competitively with Roche Diagnostic Systems, Inc., 1080 U.S. Highway 202, Branchburg, NJ, 08876-1760. Under the authority of 41 U.S.C. 253(c)(1) when there is reasonable basis to conclude that the agency's minimum needs can only be satisfied by(I) unique supplies available from only one source. This highly specialized scientific instrument is a required ingredient in established procedures or methodology in research directed toward further development by the USDA, Livestock and Poultry Institute, Beltsville, Maryland. The general area for research is the determination of the net flux of nutrients and metabolites across tissue beds of ruminants. Assay protocols include the analysis of numerous metabolites in plasma, deproteinized blood, buffer systems, and various types of cell media. While the instrument must be capable of conducting numerous enzymatic and colorimetric assays for future development, it must be capable of using pre-established protocols for the analysis of para-amino hippuric acid, glucose lactate, a-amino nitrogen, ammonia nitrogen, acetoacetate, B-hydroxy butyrate, nonesterified fatty acids, glutamate, and glutamine. In order to maintain this essential research capability, and duplication of past efforts through application of established procedures the proposed contract will include the following: Line item #1: One reconditioned bench top COBAS FARA II, Roche Centrifugal Chemistry Analyzer, 115V, 60Hz with disposable starter kit and reagent starter kit. Line Item 02: One Accessory Starter Kit which includes: Instrument Cart, Line Conditioner, RS232 Bidirectional Computer Interface, Operators Manual, Florescence Polarization Capability, 2 days on site training, 12-month warranty on parts and labor plus 1 Preventive Maintenance Visit. The Centrifugal Chemistry Analyzer's minimum capabilities are as follows: 1)Measurement Modes include Absorbance, Fluorescence, Polarization Intensity, Turbidimetry and Nephelometry 2)allow for STAT interrupt 3)130 programmable channels to define tests, profiles and ratio calculations 4)must perform the following calculations: Endpoint or linear regression analysis, Linear search for enzymes, 6 non-linear calculation modes, Statistics on raw data and results, Reaction limits and antigen excess checks, Reagent absorbance check, Sample absorbance check, Recalculation of results from raw date, Ratio calculations utilizing up to 4 tests and Toxicology cutoff check 5)must perform the following types of calibration: Theoretical factor, Linear and non-linear (up to 8 standards), Selective calibration interval, Precalibration, Single point non-linear curve correction 6)must meet the following qualifications: Bench top, Centrifugal, Cuvette capacity-30, Cuvette composition-Acrylic plastic, Path length (variable) 2-16 mm, Cuvette volume-400 ul, Read interval (minimum)-5 seconds, Temperature control -20-50 degrees C(in steps of 0.1 degrees C), Wavelength control-up to 10 per test (in steps of 1nm) 7)optical system must meet the following requirements: Type-Holographic Grating monochromator, Wavelength range 285-750nm, Wavelength accuracy-positive/negative 1 nm, Spectral half band width 5.5 nm(at 500 nm), Linear absorbance range (340 nm) 0-3A (deviation <1%), Photometric reproducibility (1 SD) 0.0009A up to 2.0 A, Photometric accuracy-as low as positive/negative 0.5% of value of 0.00001A, Light source-Pulsed xenon flashtube, Florescence Measurement-90 degrees, Photomultiplier-Solid state (200-800 nm), Bandpass Filter (Fluorescence Pol.)-505-545 nm, Bandpass Filter-430-520 nm), Polarizer: Stepper Motor controlled 8) Analysis Rate: Up to 400 tests per hour 9) pipetting systems must meet the following requirements: Type-XYZ robotic pipetting system, Sample volumes: 0.0-95.0 ul (in 0.1 ul steps), Reagent volumes: 0-370 ul (in 1 ul steps), Sample and reagent level detection: Capacitative, Pipetting time per rotor (minimum): 1 minute, Sample precision-less than or equal 1.5% (at 5 ul), Reagent precision-less than or equal 2.0% (at 150 ul), Prewetting and tip cleaning-Sample and reagent 10)must meet the following communication requirements: Keyboard-Alphanumeric, Monitor-9"CRT, Thermal dot matrix printer-Alphanumeric, 40 characters per line, Patient report-Collated report with alphanumeric patient ID and normal range flags, Ancillary reports-Raw data, statistics, abbreviated Levy-Jennings Quality Control plotting, Computer interface-Bi-directional RS-232. The delivery is FOB destination to the Beltsville Agricultural Center, Beltsville, Maryland. Delivery is required on or about April 30, 1997 or 30 days after award. Any interested/qualified source who believes they can provide the required equipment must respond by 3:00 p.m. March 28, 1997. All such responses will be evaluated in order to determine the ability to meet the above stated capabilities. The Government will award a contract resulting from this solicitation to the responsible offeror whose offer conforming to the solicitation will be most advantageous to the Government, price and other factors considered. The following factors shall be used to evaluate offers: Technical capability, past performance and price. Technical capability and past performance, when combined, are significantly more important than cost or price. Responses shall include literature, brochures and other such information corresponding to the required items which demonstrates the capabilities of the offeror's equipment. The following FAR clauses and provisions apply: FAR 52.212-1, Instructions to Offerors-Commercial Items; FAR 52.212-2, Evaluation-Award to a technically acceptable offeror, at a fair and reasonable price; FAR 52.212-3, Offeror Representations and Certifications-Commercial Items. FAR 52-212-4, Contract Terms and Conditions-Commercial Items. The following FAR clauses in paragraph (b) of FAR 52.212-5, Contract Terms and Conditions Required to Implement Statues of Executive Orders-Commercial Items, will apply to the resultant contract: 52.222-26, 52.222-35, 52.222-36, 52.222-37, 52.225-3 and 52.225-21. All offerors must include a completed copy of FAR 52.212-3 with their response. (0059)

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