Contracts Management Section, National Institute of Dental Research, NIH, Natcher Building, Room 4AN-44D, 45 Center Drive MSC 6402, Bethesda, Maryland 20892-6402

A -- CREATION OF TRANSGENIC MICE FOR STUDY OF DEVELOPMENT OF BONE, CARTILAGE, CRANIOFACIAL AND TOOTH TISSUES SOL RFP NIH-NIDR-2-97-7R DUE 043097 POC Marion L. Blevins 301 594-0652 The National Institute of Dental Research has a requirement for the creation of transgenic mice to study regulation and in vivo function of gene products involved in the formation of bone, cartilage, tooth and craniofacial tissues. The contractor must be able to create three types of transgenic mice: 1. transgenic mice expressing previously known genes and also newly identified genes in NIDR's Oral Genome Project under the control of a tissue-specific promoter or a strong general promoter (i.e. CMV and beta-actin promoters), 2. transgenic mice harboring a reporter gene (i.e. lacz gene for beta-galactosidase) under the control of various lengths of the promoter and enhancer of the genes described above, and 3. transgenic mice expressing the Cre recombinase under the control of a tissue-specific promoter. The contractor must have the capability to generate a minimum of five founding lines per construct for approximately 100 constructs to be provided by NIDR over a period of three years. See Note 1. (0059)

Loren Data Corp. http://www.ld.com (SYN# 0002 19970304\A-0002.SOL)