U.S. Environmental Protection Agency, 26 W. Martin Luther King Dr., Cincinnati, OH 45268

66 -- TWO PHOTON CONFOCAL MICROSCOPE IMAGING SYSTEM SOL PR-CI-97-10387 POC Billy Oden -- (513) 366-2126 WEB: Office of Acquisition Management -- Cincinnati Contracts Management Division, http://www.epa.gov/oam/cinn_cmd. E-MAIL: U.S. EPA -- Cincinnati Contracts Management Division, oden.billy@epamail.epa.gov. The USEPA intends to negotiate on a sole source basis, under authority of FAR 6.302, with Bio-Rad Laboratories for the acquisition of a turnkey, installed Two-photon Confocal Microscope Imaging System. The system shall be delivered with a 15 mW Krypton/Argon confocal laser with emission lines at 488, 568 and 647 nm. The single photon laser shall be coupled with a single mode polarization preserving fiber that is mounted to allow 4-axis adjustments at the output. Filters shall be included for imaging green, yellow/red and far red flourophores and reflection samples, e.g. FITC/RHOD/Cy-5. Exciter filter set includes: 488DF10, 568DF10, 647DF10 and 488/568DF10. Emission filter set includes 522DF35, OG515, 585LP, 598HQ40, and 80DF32. Filter blocks included: Triple Labeling Filter Block Set (T1 and T2A[560DCLP]), Singe excitation filter set for simultaneous imaging of FITC and Texas Red (A1/A2), Polarizing Beamsplitter (B1), one Open Block, and one block with a dichroic for uncaging using the Ti-Sapphire wavelengths allowing calcium distribution to be imaged using the Fluo-3 fluophore. For 2-photon excitation, the system shall be capable of accepting a femto-second pulsed Ti-Sapphire laser tuned to excitation wavelength raging from 690 to 950 nm. The periscope that couples the laser beams to the scan head shall allow the beam height to be moved without rotating the polarization. The system shall be equipped with combining optics for mixing the output from the two-photon and fiber-delivered confocal lasers. The basis for sole source negotiations is Bio-Rad Laboratories' proprietary rights under the world-wide licensing agreement between Bio-Rad and the patent owner of two photon confocal microscopy technology, Cornell Research Foundation, the patent and licensing arm of Cornell University. The patent number referred to in the agreement is U.S. Patent No. 5034613. THIS IS NOT A REQUEST FOR PROPOSALS. Sources wishing to be considered must submit documentation to the office identified above, which establishes the ability to furnish turnkey, two photon confocal microscope systems as licensed to Bio-Rad by Cornell Research Foundation. Documentation must be submitted within 15 calendar days of publication of this notice. (0083)

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