NIH, OPM, AB-A, EPS, Rm 894, 6120 Executive Blvd., MSC 7260 Bethesda, Maryland 20892-7260

66 -- MASS SPECTROMETER SOL 263-97-B(BN)-0032 DUE 051997 POC Ms. Foteni T. Tiffany, Contract Specialist, 301-402-3079. The National Institutes of Health, National Institute of Child Health and Human Development, Laboratory of Cellular and Molecular Biophysics requires a High Performance Floor-Standing Integrated MALDI-TOF and an Electrospray-TOF Mass Spectrometer System to enable the study of high molecular weight biomolecules, particularly proteins. This can be accomplished most effectively only by instruments able to measure molecular weights with high accuracy and able to resolve the monoisotopic ion from the main isotope cluster at masses less than or equal to 4000. The following additional information is furnished for the Mass Spectrometer: High Performance Floor-Standing Integrated MALDI-TOF Mass Spectrometer: 1) The system shall have a resolution of no less than 15,000 (m/z 1296, 5733, 8565) with a 10 ppm mass accuracy for peptide masses when internal calibration is employed. 2) The system shall employ a two filed gridded ion source with Delayed Extraction (DE) Technology to enable the acquisition of high resolution data at high mass accuracy. Delay times and voltages shall be continuously variable. 3) Shall include collision cell, gas inlet and appropriate fittings for gas connection and regulation. 4) The system shall include a 100 well sample plate capable of reproducible full X-Y control positioning and capable of automatic operation. Video camera and monitor for sample viewing shall be provided. 5) Shall have a 2 Ghz acquisition system consisting of the data system General Purpose Interface Bus, control hardware and software, and a 2 Ghz digitizing oscilloscope with 500K record length. Benchtop Electrospray Ionization (ESI)-based Mass Spectrometer: 1) Shall be a benchtop electrospray ionization (ESI)-based mass spectrometry system that provides capability for the acquisition of high resolution time-of-flight spectra. Mass range shall be selectable as 40-4000 Da and 100-10,000 Da. 2) Shall have a resolution of no less than 5,000 over entire mass range simultaneously when operating in the 40-4000 Da range. Under these conditions, mass accuracy shall be plus or minus 20 ppm using internal calibration. Attributes Common to Each Mass Spectrometer: 1) Both instruments shall be capable of positive or negative ion operation. 2) Both systems shall include all hardware and software necessary for acquisition, display, manipulation, reporting, and printing of mass spectra data files. Computers provided with the systems shall be Pentium processor based and include at least 32 Mbytes of FAM and 1 Gbyte hard disk. 3) Systems shall include software search engine optimized for searching data bases using mass measurements made at high mass accuracy. All responsible sources may submit a bid which shall be considered by the agency. (0090)

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