National Institute of Environmental Health Sciences, Contracts and Procurement Management Branch, 4401 Building, 79 Alexander Drive, P. O. Box 12874, Research Triangle Park, NC 27709

66 -- ACQUISITION OF ESI MASS SPECTROMETER WITH MS/MS CAPABILITIES SOL 273-97-P-0004 DUE 073197 POC Contact George Earle, Contracting Officer (919) 541-0377 The NIEHS is interested in purchasing an ESI Mass Spectrometer with MS/MS capability. This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in Subpart 12.6, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; proposals are being requested and a written solicitation will not be issued. The Request for Proposal number is 273-97-P-0004 and incorporates provisions and clauses through FAC 90-45. The solicitation is not a set-aside and is under SIC 3826, size 500. Delivery, installation, and training shall be made within 120 days after award of the contract. Specifications are as follows: electrospray mass spectrometer with tandem mass spectrometry capabilities. The analyzer can be quadrupole, ion trap type or time-of-flight type. It must readily interface with liquid chromatography and capillary electrophoresis. It must have negative ion capabilities and have nanospray ESI capabilities. Mass range of 2-4000 preferred but 2-1600 will be considered. Ionization source shall be electrospray; routine negative ion capabilities under data system control, including alternate positive and negative scans in MS/MS mode, e.g., selected monitoring of m/z 79 in negative ion followed by an MS/MS fragment ion scan in positive mode. Mass accuracy > 0.01%. Scan speed of at least 2 secs for a full scan of the mass range in MS/MS mode. Mass resolution of MS/MS scans -- unit resolution over mass range; sensitivity -- interpretable MS/MS spectra should be obtainable from a femtomole level peptide sample. MS/MS modes -- interpretable daughter ion spectra are of primary importance. Parent ion scanning and neutral loss scanning are also desirable. Software must be capable of detecting an LC peak, selecting a molecular ion and initiating an MS/MS scan. Inlet system must be compatible with coaxial sheath flow for use with e.g., capillary electrophoresis. There must be an integrated biological application software package including site license and foreground/background data system operation. The instrument shall be warranted for at least one year after installation and shall include training. An instrument used in a demonstration laboratory for less than one year will be acceptable; however, offerors shall certify age of demonstration equipment. Leasing may be considered, if in the best interest of the Government. Offerors may submit an alternate proposal for leasing, if so desired. Provide price and length of time price will be honored for the following accessories that may be purchased at a later date -- nanospray source, integrated HPLC system, integrated capillary electrophoresis interface and recommended spare parts kit. All offers shall be evaluated using the following evaluation criteria: 1. To evaluate the MS/MS capabilities of the instrument a standard electrospray source to provide structural information, two protein digests and one oligonucleotide will be provided for analysis. Mass resolution for fragment ions shall be better than unit and mass accuracy shall beq0.1 amu. Offerors shall report the amount of material injected and flow rate. (A) A tryptic digest of a protein that has been post-translationally modified by phosphorylation. Molecular weights of all peaks shall be determined and as much structural information as possible for each component shall be provided (positive ion). Phosphorylated peaks shall be readily identified as such by observation of m/z 79 in the negative ion mode, worth 20 points; (B) A tryptic digest of a protein that has been post-translationally modified by one or more dibromonitrosobenzenesulfonic acid groups. Molecular weights and as much structural information as possible for each component shall be provided, worth 20 points; (C) An oligonucleotide analyzed in the negative mode, worth 20 points. 2. To evaluate sensitivity, MS/MS spectra of renin substrate terradecapeptide (RSTP) are to be acquired at five different concentrations using flow injection analysis and a standardelectrospray source. A maximum of two microliters is to be consumed during data acquisition, Solution A: 1 picomole/microliter; Solution B: 100 femtomole/microliter; Solution C: 1 femtomole/microliter; Solution D: 100 attomole/microliter. If satisfactory results are obtained with a low concentration sample, the higher concentration samples need not be run, worth 20 points. 3. Software evaluation -- all data acquired during the tests are to be provided with the offer to the NIEHS Contracting Officer along with a complete set of data manipulation programs to be installed (temporarily) on NIEHS computers. The software shall be evaluated based on ease of use, ease of instrument set-up and calibration, range of data presentation modes, accuracy and extent of software tools for analysis of data from the biological samples. For the latter, such utilities as identification of fragmentation patterns, automated data work-up and identification of proteins from libraries based on tryptic maps/fragmentation data will be evaluated, worth 20 points. Total maximum technical score is 100 points. Twenty points will be awarded to the lowest priced technically acceptable offer. Other technically acceptable offers will be given a proportional share. Technical factors will be given primary consideration; however, if two or more offerors are considered technically equal, price will be the determining factor. All firms wishing to submit an offer will be furnished samples by overnight courier for analysis on the type of instrument proposed. Samples will be released at one time. Offerors are responsible for all samples after arrival. Requests for samples must be received not later than close of business 21 April 1997. Samples will be released 24 April 1997. The following FAR provisions apply to this acquisition; 52.212-1 Instruction to Offerors-Commercial and 52.212-2, Evaluation-Commercial items. There are no additional addenda. Offerors shall include a completed copy of the provisions at 52.212-3, Offeror Representations and Certifications-Commercial (Jan 1997) with offers. FAR clause 52.212-4, Contract Terms and Conditions-Commercial (Aug 1996), applies to this acquisition. The following sections of paragraph (b) of 52.212-5 Contract Terms and Conditions Required to Implement Statutes or Executive Orders- Commercial Items (Aug 1996) apply: (1) 52.203-6, Restriction on Subcontractor Sales to the Government with Alternate I; (2) 52.203-10, Price or Fee Adjustment for Illegal or Improper Activity; 52.219-9; (6) 52.222-26, Equal Opportunity; (7) 52.222-35, Affirmative Action of Special Disabled And Vietnam Era Veterans; and (9) 52.222-37, Employment Reports on Special Disabled Veterans and Veterans of the Vietnam Era. The successful contractor shall provide, at no additional cost, installation and training to NIEHS designated employees. There are no CBD numbered notes that apply to this notice. Offers are due not later than close of business 31 July 1997. Facsimile proposals will not be accepted. For information concerning this solicitation contact George Earle, voice (919)541-0377, FAX (919)541-2712. (0099)

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