DEVELOPMENTS AT NIST Researchers at the National Institute of Standards and Technology (NIST) have developed a number of new devices and methods involving a variety of technologies. In certain cases other parties have participated in the development of these technologies. Anyone interested in the further development of any of these technologies or in applying for a license to commercialize these technologies should send a written request for further information, referencing the NIST Docket Number and Title, to: National Institute of Standards and Technology, Industrial Partnerships Program, Building 820, Room 213, Gaithersburg, Maryland 20899; Telecopy: 301-869-2751. This is not an announcement of a contract action or a grant. NIST DOCKET NUMBER: 96-045PA Title: Electroenzymatic Reactor For Biocatalytic Hydroxylation Description: Interest in biocatalytic hydroxylation derives from its ability to transform organic substrates having no functional groups into oxygen-bearing compounds with high regio-or stereo-selectivity. Use of redox enzymes in these syntheses is hampered by intrinsic dependence on stoichiometric amounts of freely dissociated cofactors, such as NADH and/or redox partner proteins, which supply necessary reducing equivalents. Economic feasibility requires that simple, effective and stable methods for cofactor recycling be found. Electrochemical cofactor regeneration can meet these requirements. Previously, a bioelectrochemical process has been described in which electrons are transferred directly (without mediators) between an electrode and redox-active biological material, such as an enzyme or protein. In that work, electron transfer was achieved using various modified metal or graphite electrodes. Such processes suffer from either inefficiency (low redox reaction rates) or rapid decline in activity due to component fouling by proteins. In the present disclosure, the P450 enzymatic cycle, which requires a continuous supply of reducing equivalents, molecular oxygen and an amenable organic substrate, is utilized in an unique electroenzymatic reactor to catalyze the generation of sterochemical hydroxylation products. The reactor permits rapid and persistent electron transfer to a P450 protein cofactor (putidaredoxin) by using certain tin oxide or iridium oxide cathodes, while simultaneously providing necessary dissolved oxygen at platinum or ruthenium oxide counter electrodes. The need for NADH and the redox protein, flavin reductase, which are required in the native cycle, has been eliminated. NIST DOCKET NUMBER: 97-016PA Title: Process For Enhanced Atomization, Vaporization And Combustion Of Liquid Fuels Using Oxygen-Enriched Atomization Air Description: A process, herein referred to as the 'MARNIOX' process, is described in which control of composition of the atomization gas in twin-fluid atomizers is used to enhance atomization, vaporization and combustion of liquid fuels. The potential benefits associated with this process include improved flame stability, combustion efficiency and intensity, and reduced harmful pollutants to the environment. WEB: NIST Contracts Homepage, http://www.nist.gov/admin/od/contract/contract.htm. E-MAIL: NIST Contracts Office, Contract@nist.gov.

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