National Institutes of Health, National Heart, Lung, and Blood Institute, Contracts Operations Branch, Procurement Section, 6701 Rockledge Drive, RKL2 Building, Room 6143, Bethesda, MD 20892-7902

66 -- BIOSPECTROMETRY WORKSTATIONS SOL NHLBI-HL-P-97-119 DUE 080497 POC Gina M. Cianflone, Contract Specialist, (301) 435-0363; Kathleen J. Van Steelant, Contracting Officer (301) 435-0364 This is a synopsis for commercial items, prepared in accordance with the format in FAR subpart 12.6, as supplemented with additional information included in this notice. The Laboratory of Biophysical Chemistry, National Heart, Lung, and Blood Institute (NHLBI) of the National Institutes of Health (NIH) has a requirement to obtain two (2) biospectrometry workstations. The technical specifications are as follows: Specifically, Biospectrometer A shall include the following features: Shall be a matrix as sisted laser desorption mass spectrometer with reflectron and delayed extraction, having resolution (m/ m, where m is FWHM)better than 15000 at m/z 3500 in reflector mode. Masses at m/z from 500 to 6000 shall be baseline resolved simultaneously. Mass accuracy shall be better than 10 ppm from 500 to 6000 and PSD fragment measurements from 50 to 3000 by internal calibration. Post source decay (PSD) shall be performed with fully automatic computer control and PSD ions shall produce bn and yn ions. Mass selection for PSD experiments shall be done with better than 150 resolution at 1500. MS measurements shall be performed both at linear and reflector modes with a sensitivity better than sub-picomole for peptides, protein and DNA. MS shall be measured in both positive (25kV) and negative (20kV) selected by mouse click. The flight path shall be > 2 m in linear mode and >3m in the reflector mode. The spectrometer shall be operated under a Windows or Windows 95 PC and it shall be Internet ready. The detector shall be low mass gate controlled. The vacuum system shall be pumped by at least two 2 40 l/s turbo pumps. A 2GHz digital scope shall be included for maximum mass resolution and mass accuracy. A sample plate holding at least 100 samples shall be supplied and viewable during operation with a 100 X video camera and the plate shall be fully movable in XY position. Data base search software shall be provided for identifying proteins from peptide masses and PSD spectra. 2) Specifically, Spectrometer B shall include the following features: The spectrometer shall be a electrospray/time of flight m ass spectrometer equipped with a reflectron and all hardware and software necessary for data acquisition and reduction of mass spectral data files on a chromatographic time-scale. It shall have mass accuracy of +/- 20 ppm for peptides with masses < 2000 Da using an internal standard. Singly charged ions from m/z 40-4000 shall be detectable . Ions are to be measured at 10kHz intervals orthogonal to their original trajectory. The resolution shall be > 5000 FWHM and detection shall be by a channel plate detec tor where the signal is measured by a 1GHz time to digital converter. The system shall be turbo pumped and operate at a 3-4 kV acceleration potential and be capable of detecting both positive and negative ions. The data system shall be at least 166MHz, 32 Megabyte RAM, 1 Gigabyte disk and 17 inch monitor. Software upgrades shall be provided free for at least 2 years, and warranty shall be for at least 90 calendar daysfrom delivery. This solicitation is not a set aside. The applicable Standard Industrial Classification (SIC) is 3821, size standard 500 employees. The proposals may be due fifteen calendar days from the publication date of this synopsis. Interested parties should submit a written request (via mail or facsimile transmission to (301) 480-3345) for a copy of the solicitation number NHLBI-HL-P-97-119 to the National Heart, Lung, and Blood Institute, Contracts Operations Branch, Procurement Section, Building RKL2, Room 6150B, 6701 Rockledge Drive, Bethesda, Maryland 20892-7902, Attention: Gina M . Cianflone. All responsible sources may submit a proposal, which if timely received shall be considered by the agency. See Numbered Note(s) 12. (0195)

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