National Institutes of Health, Office of Procurement Management, 6120 Executive Blvd., (EPS), Rockville, Maryland 20892-7260

66 -- INVERTECD ULTRAVIOLET (UV) CONFOCAL MICROSCOPE SOL 263-97 -- BB(GD)0049 DUE 090297 POC Donald Wilson, Contract Specialist (301) 402-2849 This is a combined synopsis/solicitation for commercial items, prepared in accordance with the format in FAR Subpart 12.6, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; bids are being requested and a written solicitation will not be issued. The solicitation number 263-97-B(GD)0049 bid (IFB). The solicitation/contract will include all applicable provisions and clauses in effect through FAC 90-45. Item to be acquired: Provide an Inverted Ultraviolet (UV) Confocal Microscope System NT based system. The System shall include upgrade to Multiband Confocal Spectrophotometry, permitting continuous adjustment of emitted wavelengths and providing for optimal spectral detection and separation, as well as determination of spectral emission, quantity 1. Delivery F.O.B. destination National Institutes of Health, Building 10,Room 4A26, Rockville, MD 20892. No later than 120 days after contract award. FAR 52.212-1, Instructions to Offerors-Commercial Items, applies to this acuisition. Award will be made to the lowest priced, technically acceptable offeror. Provide descriptive literature and owner's manual with maintenance procedures as part of the bid. FAR clause 52.212-4, Contract Terms and Conditions-Commercial Items, applies to this acquisition. The following FAR clauses in paragraph (b) of FAR 52.212-5, Contract Terms and Conditions Required to Implement Statutes or Executive Orders-Commercial Items, will apply to the resultant contract: 52.203-6, 52.214-26, 52.214-27, 52.214-29, 52.219-8, 52.222-26, 52.222-35, 52.222-36, 52.232-25, 52.245-2, 52.249-4, 52.249-8, 52.246-2, 52.246-4, 52.246-16, 52.252-2, 52.212-2, 52.232-28. The Statement of Work is as follows: C. The Inverted UV Confocal Microscope NT based system shall include the following salient characteristics and features and shall also provide for the functions as follows: 1. Scan Module with 4 Fluorescence Reflection Detectors (Photomultipliers) for 4 channel detection. 2. 3 Laser Configuration with Acousto Optical Tuneable Filter (AOTF), 4 Channel Filterset for 4 Channel Scanner Head. Ar and Ar-Kr lasers fiber-optically coupled to a merge module (to eliminate beam wander). Merge module fiber-optically coupled to the Scan-head. The following lasers, all with a minimum output of l0 mW, are to be provided: Argon (Ar) Laser (488 nm wavelength). Argon-Krypton (Ar-Kr) Laser (568 nm wavelength). Helium/Neon (He-Ne) Laser (633 nm wavelength). 3. UV Option with Argon -- UV Laser, with UV filterset. 4. UV Laser fiber-optically coupled to the Scan-head. UV-Visible Scanner Optics with the UV coupled via a separate lightpath with a separate source-pinhole, to insure exact co-excitation and co-alignment of UV and Visible illumination. 5. Panel-Box with 7 Digital Potentiometers for online adjustment of scanning parameters. 6. Multitasking High Performance Windows NT based Workstation. A. Pentium-Pro (P6)/200MHz, 128 MB Tower Crate configuration. B. 2.1 GB SCSI Hard Disk, 3.5 inch Floppy Drive, 4XCD. C. High Performance Frame Grabber, 3Com Ethernet board, SCSI controller. D. 2 each, 21 inch Image Monitors, True Color, total 2560 x 1024 Pixels. E. MS Keyboard, Mouse, internal Data Modem 28.8 kbps. F. MS Windows NT 4.0, Excel NT 7.0. G. Simultaneous Acquisition of 4 Data Channels at 1024 x 1024 Pixels Resolution. H. NT Scan Driver Electronics and System Controller for Up to 4 channels I. Scanning Software for Scan Control, Display and 2D Image Processing. J. TCP/IP Software, On-line Software and Hardware Manuals. 7. 3D Software Package, Stereo, Rotations, 3D and Slicing, Raytracing, Volume rendering, and Simulated Fluorescence Process 8. 2D Image Analysis Software Package 9. Physiology Software Package. 10. Galvanometer driven Z-stage with rotating stage insert, with Z motion precisely controlled by a galvanometer with a minimum step size of 10 nm and a reproducibility better than 40 nm. 11. The System shall include upgrade to Multiband Confocal Spectrophotometry, permitting continuous adjustment of emitted wavelengths and providing for optimal spectral detection and separation, as well as determination of spectral emission. 12. True Confocal Optical Path with one excitation pinhole for the Visible light-path and a separate excitation pinhole for the UV light-path. Both pinholes are to serve as alignment points for the system to prevent erroneous sample excitation due to laser misalignment. 13. One computer controlled detector pinhole (Variable) for all fluorescence and reflection detectors, to insure exact localization of multiple flourochromes with no pixel-shift between channels. 14. Scanner user switchable between Upright and Inverted Optical Microscopes, with a 5 minute conversion time and no re-calibration or re-alignment required. 15. Guaranteed resolution of 180 nm in the x -- y axis, and 350 nm in the z -- axis. 16. Scanner linearity better than 99%, and a miniaturized Optical Path with a length of 20 cm or less, for long term stability and reduced realignment need, leading to experimental consistency. 17. Optical Transfer Efficiency of 0.92 or better. 18. Fully automated and computer controlled excitation, dichroic, and barrier filters. All dichroic beamsplitters and barrier filters field upgradeable for ease of servicing and reduced loss of research time 19. Inverted UV Research Microscope, with Trinocular tube. 20. Motorized coarse and fine focus and motorized 6 place focusing nosepiece. 21. Infinity corrected optical system with built-in Bertrand lens. 22. Integrated power supply for Transmitted Light Illuminator. 23. PMT based detector for transmitted light. 24. Incident light fluorescence outfit. 25. Microscope Objectives as follows: PL APO 40x/1.25-0.75 Oil PL APO 100x/1.40-0.70 Oil PL Floutar 10x/0.30 PH 1 PL APO 40x/1.25 Oil PH 3 PL APO 100x/1.40 Oil PH 3 PL APO 20x/0.60 26. Differential Interference Contrast All offerors must include a completed copy of FAR 52.212-3, Offeror Representations and Certifications -- Commercial Items. SIC code is 3821, size standard is $3.5 million. See numbered Note 26. Offers are due by 10:00 a.m. local time, September 2, 1997. (0227)

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