National Institute of Health, National Institute of Diabetes & Digestive Kidney

B -- B- SERVICE FOR THE DEVELOPMENT OF A MOUSE KNOCK-IN MODEL FOR FRAGILE X SYNDROME SOL RFQ-97-305 DUE 091597 POC Desiree Kelly, Purchasing Agent 301-594-7731. The National Institutes of Health (NIH) National Institues of Diabetes and Digestive and Kidney Diseases has a requirement for the development of a Mouse Knock-In for fragile X syndrome on a competitive basis. The fragile X mental retardation from in humans is due to an expansion of CGG trinucleotide repeats within the FMR1 gene which results in the absence of the FMR1 protein (FMRP). We would like to develop a mouse model for the triplet expansion defect by inserting a large number of CGG repeats into the CGG tandem array in the 5 UTR of the mouse FMR1 gene. The following milestones must be achieved: restriction mapping and exonic sequencing of the mouse FMR1 genomic clone, engineering of the targeting vector. We will provide a fragment of multiple (>100) CGG repeats that will need to be put into the targeting vector, Transfection of the targeting vector into ES cells and selection of at least 150 ES clones, Screening for homologous recombination by southern analysis of selecting ES clones. If no recombination then steps 3 and 4 will be repeated a second time at no charge to us, Expansion of recombinant clones, and Production of chimeric animals (at least 3), which will all be shipped to us after having been MAP tested for pathogens. All responsible sources may submit a quotation, which if timely received, shall be in within the Simipified Acquisition Thereshold $ 100,000. It is anticapted that Request for Quotation (RFQ) NIH-NIDDK-97-305 will be available fifteen (15) days after the publication date of this synopsis receipt of quotations will be due ten (10) days after the release of the RFQ. The anticapted award date is September 26, 1997. Requests for copies of the RFQ must be in writing to the National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) The Natcher Building, Room 6an32, 45 Center Drive MSC 6600 Bethesda, MD 20892-6600 Attention: Desiree Kelly FAX quotations will only be accepted if dated and signed by an authorized company representative by 3:00 pm on September 24, 1997. (0241)

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