National Cancer Institute, Research Contracts Branch, Cancer Etiology Contracts Section, Executive Plaza South, Rm. 620, 6120 Executive Blvd., MSC 7224, Bethesda, MD 20892-7224

B -- PROCUREMENT OF DNA, RNA, TRANSFORMED LYMPHOCYTES, AND LYMPHOBLASTOID LINES FOR GENETIC STUDIES SOL N02-CP-81019-50 DUE 120197 POC Karen L. McFarlane, Contracting Officer, (301) 496-8611 WEB: National Cancer Institute, http://cbdnet.access.gpo.gov. E-MAIL: Karen L. McFarlane, Contracting Officer, NCI, km63k@nih.gov. The Genetic Epidemiology Branch (GEB) of the Divison of Cancer Epidemiology and Genetics (DCEG) of the National Cancer Institute (NCI) is recompeting a project which is currently being performed by the American Type Culture Collection under contract No. NO2-CP-40500. The GEB is seeking a Contractor who will support the GEB by performing four assays or procedures. One, two, three, or all four of the procedures will be performed on each biospecimen. The procedures are: 1) Extraction of DNA from fresh whole blood specimens, transformed lymphoblastoid lines, cultured fibroblasts, tumor specimens, buccal cells, paraffin-embedded tissue, Guthrie cards, or cytology preparations; 2) Extraction of RNA from fresh whole blood specimens, transformed lymphoblastoid lines, cultured fibroblasts, tumor specimens, or other biological specimens, as possible from advances in technology; 3) Isolation of lymphocytes, and transformation of lymphocytes to lymphoblastoid cells using the Epstein-Barr virus (EBV); 4) Expansion of lymphoblastoid cell lines up to gram quantities. Approximately 5200 samples will be submitted to the Contractor for processing during a one-year period. It is anticipated that an incrementally funded, cost-reimbursement, completion type contract will be awarded for a three- year period, with two one year options. The anticipated RFP date is on or about October 30, 1997, with responses due approximately 30 calendar days after the actual issuance date. (0279)

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