U.S. Environmental Protection Agency, 26 W. Martin Luther King Dr., Cincinnati, OH 45268

66 -- DUAL-LINE LASER FLUORIMAGING SYSTEM SOL PR-OH-98-00127 POC Sharon Clement (513) 487-2030 WEB: Office of Acquisition Management -- Cincinnati Contracts Management Division, http://www.epa.gov/oam/cinn_cmd. E-MAIL: Sharon Clement -- Cincinnati Contracts Management Division, clement.sharon@epmail.epa.gov. The USEPA intends to award a purchase order to Molecular Dynamics, Inc. on a sole source basis for the acquisition of a turnkey dual-line Laser Fluorimaging System. This fluorimaging system shall be delivered configured with a multi-purpose dual excitation laser induced fluorescence scanner allowing for sample components to be readable directly from gels, membranes, TLC, or micro titre plates when labeled with a wide variety of single and dual fluorescent labeling protocols, and have sensitivity to not less than 1.0 fmole fluorescein end-labeled DNA in polyacrylamide gel. Data acquisition electronics shall be calibrated automatically to ensure run-to-run repeatability. Scan time shall be less than 2 minutes for 20x25 cm scan area for single color at standard resolution and less than 30 minutes for dual-color scan at high resolution and high sensitivity. The system shall include software which controls scanning, multiple color separation, image display and tools for both quantitative data analysis and final reports that is compatible with EPA's existing software and equipment. Additionally, the fluorimaging system shall have 5 orders of magnitude dynamic range, digital signal values storage in log format for greater intensity discrimination of low signal values, conversion utility for compatibility of image files with Macintosh or PC-based system desktop publishing programs, applications software for display and results analysis, and automated 1-D electrophoresis analysis software. The associated computer work station shall minimally consist of an Intel Pentium Pro 200MHz, 32MB EDO RAM, 256 KB external cache, 2GB or larger EIDE hard drive, 8X or faster multi-session IDE CD-ROM drive, 1.44 MB 3.5" diskette drive, ISA expansion slots, PCI expansion slots, shared PCI/ISA expansion slot, 17" SVGA monitor, PCI local bus SVGA video accelerator card adapter with 2MB RAM, 3Com 10/100 Base-TX Fast Etherlink network adapter, SCSI interface card, 104-key keyboard, two button mouse, Windows NT 4.0 or higher, MS Office Pro, and image analysis and display software. This action is being pursued on a sole source basis because Molecular Dynamics, Inc., is currently the only source known of commercially available equipment which will interface directly with EPA's existing family of machines (Phosphoimager, Densitometer, and Fluorimager), workstations, and software platform (ImagequaNT, FragmeNT). Any firm which feels it is capable of meeting these requirements may submit technical documentation to establish its ability to furnish the turnkey dual-line laser fluorimaging system which is fully compatible with EPA's existing equipment and software. Only those firms which submit such technical documentation will be considered. Written documentation must be provided on or before February 6, 1998 and may be mailed to 26 W. Martin Luther King Drive, Cincinnati, OH 45268. Failing receipt of the requested technical proof, USEPA will proceed to negotiate with Molecular Dynamics, Inc. for the cited system. See Note 22. (0012)

Loren Data Corp. http://www.ld.com (SYN# 0319 19980115\66-0002.SOL)