Acquisitions Management Operations Branch, NIAID, 6003 Executive Blvd, Solar Building, Room 1C38, Contracts Section, Bethesda, MD 20892-7605

66 -- LIQUID CHROMATOGRAPHY EQUIPMENT SOL NO1-9801 DUE 051598 POC Steve Gunn,301-496-2814,Tom McFadden,Contracting Officer,301-496-9762 This is a combined synopsis/notice of intent to procure commercial items prepared in accordance with the format in subpart 12.6, as supplemented with additional information included in this notice. The National Institute of Allergy and Infectious Diseases intends to procure a proprietary Liquid Chromatography System as manufactured by Amersham Pharmacia Biotech. The line items are: (1). AKTA Explorer 100A (Custom) (2). Frac-901 Fraction Collector (3) Computer Compaq 233 System with Software/configuration desktop case,233 mhz intel pentium mmx processor, 32 MB ram, 256 KB cache, 3.2 GB hard disk, 3.5" floppy drive, 16m CD-ROM drive, 104 key enhancer keyboard, PS/2 style mouse and pad, 1 serial port, 1 parallel port, compaq v70 17" SVGA monitor, IBM OS/2 Warp 4.0 (CD-ROM) version preloaded. (4) Superloop 10ml (5) Superloop 50ml Delivery shall be to the National Institutes of Health, 9000 Rockville Pike, Bethesda, Md . 20892, F.O.B. destination. The proposed procurement is critical to the multiple projectrelated to the understanding of viral diseases and the development of applicable antiviral therapies. The required Liquid Chromatography System shall be capable of the following Specifications: (1). Completely integrated and compatible with the existing chromatography systems FPLC and Pharmacia Smart system) presently in use in the laboratory for the ability to utilize extensive column reagents, software, and data/experimental protocol exchange. The ability to integrate these systems will be invaluable both for reagent expenditures/expenses and for research personnel time/efforts. (2) A flow rate range of .01-100ml/min. (3) Consist of a 3 pump intergrated system with a 2 pump dynamic mixing gradient system. (4) Motorized valves accommodating at least 7 columns, (5) Automated reverse flow capability for affinity and column cleaning. (6) Containing 2 on-line air sensors for preventing air entering colums from sample or buffers. (7) Control and record 3 UV wavelength simultaneously monitor the wavelength in therange of 190-700nm. (8) Electric pumps without the need for gas cylinders due to space and safety considerations. See number Note 22. The provision at 52.212-2,Evaluation-Commercial Items,applies to this acquisition. The government will award a fixed price purchase order to the responsible contractor,price and other factors considered: (a) Delvery of item in 45 days. (b) Prior experience in purification of proteins. All evaluation factors other than price, when combined are significantly less important than the price. The clause at 52.212-4, Contract Terms and Conditions-Commerical Items applies to this acquisition. The clause at 52.212-5, Contract Terms an Conditions Required to Implement Statues or Executive Orders-Commercial Items applies to this acquisition. The clauses of subpart (b) which apply are: 6 and 10. Any response are due no later than 3:00 P.M. Eastern Standard Time, May 15, 1998 To Steve Gunn. For Delivery through the postal service the address is rm 1c38, Solar Building, NIH/NIAID, 6003 Executive Blvd., Rockville, Md 20852. Electronic submission will not be accepted. Request for any information concerning this requirement are to be referred only to Mr. Steve Gunn, 301-496-2814. Collect calls will not be accepted.l (0082)

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