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A -- A CONSTRUCT A LARGE INSERT BACTERIAL ARTIFICIAL CHROMOSOME (BAC) LIBRARY FOR THE PORCINE GENOME. SOL RFP-14-82HW-98 DUE 043098 POC Dawn Reichenberg Procurement Clerk Fax 970-229-5531. The Agricultural Research Service intends to negotiate on a sole source basis with Roswell Park Cancer Institute, Human Genetics Department, Buffalo, New York to construct a large insert Bacterial Artificial Chromosome (BAC) library for the porcine genome. The porcine BAC library will consist of approximately 200,000 distinct clones, with the average cflone carrying at least 150 kilobasepair (kbp) procine DNA fragments. This number of cloned base pairs represents at least 10-fold coverage of the porcine genome, assuming a genome size of three billion base pairs, and assuming a relatively small proportion (<2%) of clones lacking porcine DNA. The library will be provided in the form of individual clones contained in single wells of approximately 500 microtiter dishes (384 wells per dish). The library producer will not retain any rights to the results of the research that utilizes the clones in the BAC library, nor will prevent the publication of these results by the Agricultural Research Service. The library producer will retain all rights to duplicate and/or distribute the library. Library user has right to copy the library for the purpose of maintaining the library and for use in the research programs. Published research that utilizes library clones will acknowledge the contribution of the library producer. The library users will apply various methods to screen the library for clones that contain chromosomal pieces of interst, for example clones carrying genes that are thought to play important roles in affecting production traits, or carrying markers lying close to such genes. The clones will then be used in numerous (important to allow agricultural research to use the vast amount of information being generated in the human genetics and biomedical fields), the identification of novel genes that affect production traits, the characterization of these genes, and the construction of physical maps of chromosomal regions affecting aspects of livestock production. Roswell Park Cancer Institute is uniquely qualified to perform this project. Roswell Park Cancer Institute has developed BAC libraries for other species (human, mouse, rat, canine, baboon, chimpanzee and bovine gene) and can apply the technology to rapidly develop the porcine BAC library. (0091)

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