U.S. Department of Commerce/National Oceanic and Atmospheric Administration/OFA/AGFS/AMD -- OFA51, 1305 East West Highway -- Station 7604, Silver Spring, Maryland 20910

B -- BIOASSAY OF TOXICITY OF SEDIMENT SAMPLES SOL NCNH2-12 DUE 043098 POC Sally Huber 301-713-0855, Lawrence Frazier 301-713-0851 B -- The U.S. Dept of Commerce/NOAA/NOS intends to negotiate on a sole source basis with Columbia Analytical Services, Carlsbad, CA., Using Simplified Acquisition Procedures to provide services for 1 analyzing sediment samples using the P450 Reporter Gene System bioassay, 2. Performing chemical analyses on a selected few samples that show unusally high RGS responses. The sediment samples will be collected by NOAA and its collaborators as part of sediment toxicity studies in Puget Sound, NY/NJ Harbor System and Chesapeak Bay. The RGS analysis involves testing for toxicity of contaminate sediments on the basis of the luminescence produced by a genetically engineered cell line know as cypla1-Luciferase gene construct, this cell line is proprietary and can only be used under license. Columbia Analytical Services currently holds the exclusive licenses to use it on environmental samples. Offers that have the ability to provide the above support are invited to submit information as to their capabilities and other pertinent data to the address above, to be received no later than 15 days after the date of this notice. No solicitation documents exists, therefore a request for such a document will not be considered. Reference. SJH 8012 (0107)

Loren Data Corp. http://www.ld.com (SYN# 0017 19980421\B-0006.SOL)