Acquisitions Management Operations Branch, NIAID, 6003 Executive Blvd, Solar Building, Room 1C38, Contracts Section, Bethesda, MD 20892-7605

65 -- PRODUCTION OF MONOCLONAL ANTIBODY SOL NO1-9806 DUE 061198 POC Steve Gunn, 301-496-2814, Tom McFadden, Contracting Officer 301-496-9762 This is a combined synopsis/notice of intent to procure commerical service prepared in accordance with the format in subpart 12.6, as supplemented with additional information included in this notice. The National Institute of Allergy and Infectious Diseases intends to procure a service to produce custom monoclonal antibodies developed by Spring Valley Laboratories Inc. The line items are: (1) Cell line expansion (10 power 6 to 10 power 8 cells) (2) Supernatant fluid production (500ml) (3) Cryopreservation (10 tubes of 10 power 7 cells each) (4) Liquid nitrogen storage (1 box per week) Ascites Production (1) 5 Mices: estimated yield 15 to 25 ml (2) 20 Mice: estimated yield 60 to 100 ml (3) 50 Mice: estimated yield 150 to 250 ml (4) 100 Mice: estimated yield 300 to 500 ml (5) 250 Mice: estimated yield 705 to 1250 ml Monoclonal Antibody Development (1) Monoclonal start-up charge (2) Phase 1 (immunization) (3) Phase 2 (fusion) (4) Phase 3 (cloning) per hybridoma/plate (5) Isotype determination clone per well (6) Phase 4 (ascites in five mice) per clone (7) Western blot assay (first clone) (8) Western blot assay (each additional clone) Delivery shall be to the National Institutes of Health,9000 Rockville Pike, Bethesda, Md.20892, F.O.B. destination. The proposed procurement is critical to a project related to the investigation using the two hybrid system and modified protein interaction screen to identify proteins of importance in two distinct pathways of cell death. See number Note 22. The provision at 52.212-2, Evaluation-Commerical Items, applies to this acquistion. The government will award a fixed price purchase order to the responsible Contractor. Price and other factors considered: (a) Delivery of item in 30 days. (b) Quality of monoclonal antibody purified. (c) Price All evaluation factors other than price, when combined are signficantly less important than the price. The clause at 52.212-4, Contract Terms and Conditions-Commercial Items applies to this acquisition. The clause at 52.212-5, Contract Terms and Conditions Required to Implement Statues or Executive Orders-Commercial Items applies to this acquisition. The clauses of subpart (b) which apply are : 6 and 10. Any responses are due no late than 3:00 P.M. Eastern Standard Time, June 07,1998 To Steve Gunn. For Delivery through the postal service the address is room 1c38, Solar Building, NIH/NIAID, 6003 Executive Blvd., Rockville Md. 20852. Electronic submission will not be accepted. Request for any information concerning this requirement is to referred only to Mr. Steve Gunn, 301-496-2814. Collect calls will not be accepted. (0131)

Loren Data Corp. http://www.ld.com (SYN# 0265 19980513\65-0002.SOL)