National Cancer Institute, Research Contracts Branch, PSAS, 6120 Executive Blvd, EPS/Room 638, Bethesda, MD 20892-7227

A -- RESEARCH & DEVELOPMENT SOL RFQ-NCI-80143 DUE 061998 POC Cynthia Brown, Purchasing Agent, 301-402-4509, Todd Cole, Contracting Officer The National Cancer Institute(NCI), Division of Clinical Sciences, Medicine Branch proposes to procure the services of a molecular biologist, Jana Bodorova, 443 Coll Parkway, Rockville, Md. 20850. These services are needed for a Study of Immunodeficiency in Cancer. Background: The Section of Transplantation Therapy is involved in a broad, collaborative study of the immunodeficiency associated with cancer and its treatment. The study seeks to characterize selected aspects of immunodeficiency both by direct studies and by building a broad collaborative effort. The goal is to characterized selected components of immunodeficiency associated with cancer and cancer therapy. Specifically, the selected components of immunodeficiency and their respective aims are: 1) Deficits of T cell and NK cell populations -- to investigate the biology of their generation, function, and death and 2) Application of high through-put chip technology to enhance investigations to allow multi-parameter analysis. Requirements: This research requires the services of a molecular biologist with expertise and experience in cell manipulation and molecular biology for the analysis of T cell and NK regeneration following chemotherapy and in the application of chip technology to study the immunodeficiency associated with cancer therapy. Development of chip technology and data collection and analysis are essential to these studies. Likewise, the molecular biologist shall 1) process cell and serum samples from the patient study; 2) prepare T cell populations from PBL; 3) coordinate distribution of cell and serum samples to multiple collaborating investigators; 4) characterize cell populations by FACS analysis; 5) characterized gene constructs intended for transfection of T cells and NK cells in studies of regeneration; 6) establish conditions for high transfection efficiency in T and NK cell populations; 7) carry out DNA sequencing in support of transfection studies; 8) develop alternative cell marking approaches; 9) study cell surface molecules by immunoprecipitation and western blotting; 10) investigate gene expression by RT-PCR; 11) apply chip technology for the study of immunodeficiency, including review and selection of genes of interest; and 12) establish and work with individuals involved with technology, preparation of cells for analysis, carring out analysis, and interpreting analysis. This notice is a combined synopsis/solicitation and NCI does not intend to issue a separate solicitation. This solicitation, RFQ-NCI-80143, is set-aside for small business with a Standard Industrial Classification Code of 8733. Jana Bodorova is the only source know to NCI that can meet these requirements. However, if any interested party believes it can meet the above specification, it may submit a statement of capabilities. Information furnished must be in writing and must contain material in sufficient detail to allow NCI to determine if the party can perform the requirement. Capability statements must be received in the contracting office within 15 days(06/19/98) after the publication date of this notice. A determiniation by the government not to compete this proposed contract based upon responses to this notice is solely within the discretion of the government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. (0154)

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