National Institutes of Health, National Heart, Lung, and Blood Institute, Contracts Operations Branch, 6701 Rockledge Drive, Room 6100, MSC 7902, Bethesda, MD 20892

66 -- INSTRUMENTS AND LABORATORY EQUIPMENT SOL NHLBI-PS-98-301 DUE 063098 POC Cecilia D. Moarales, Purchasing Agent, (301)435-0369, Debra C. Hawkins, Chief, Procurement Section, (301)435-0366 The National Heart, Lung and Blood Institute (NHLBI) intends to purchase the brand name only items listed below. The followng six items are manufactured by Carl Zeiss Inc.: 1) One (1) each, part number 4513039901 Axiovert S100 TV Microscope stand with TV port pointing downwards (split ratio 100% visual/ 100% Video); objective nosepiece 5X H/DIC, and with integrated 4X turret with bertrand lens, two free positions must be available for the optovar inserts 1.6X (451373) and 2.5X (451374). 2) One (1) each, part number 4513759901 Lateral TV adapter for Axiovert 100/135 with 2-position beam selector for either 100% visual, or 30% VIS/ 70% video. Integrated beam splitter interchangeable with any commercially dichroic beam splitter, the required size is 26MM X 36MM X 1MM. 3) One each, part number 451356 Long working distance condenser NA 0.30 for axiovert 100/135 -- working distance is 70MM, Max. Illuminated field = 8MM; for brightfield with adjustable aperture iris diaphragm, and with annular phase diaphragms PH 2; suitable for brightfield objectives with NA -/< 0.42, in PH 2 for objectives with NA up to 0.75. Attachment objective 0.55 (only for brightfield) for objectives with NA-/< 0.60 (40X magnification). 4) One each, part number 451383 EPI-Fluorescence equipment FL for axiovert 100/135, with High intensity FL excitation from 360NM for totally homogeneous object field illumination with adjustable and centerable field iris diaphragm, 3-position slider with open position, open position with BG38 red suppression filter, and closed position. Shall accept all zeiss HBO/XBO and Gak100 illuminator systems. 5) two (2) each, part #3800799540 Halogen Bulb 12V, 100W, for LH 447217. 6) One (1) each, part number 440931 CP-Achromat 10X/0.25PH1, WD=5.1. 7) Two (2) each Mercury burner HBO 100W DC /L2 manufactured by Ushio. 8) (The following three items are manufactured by Chroma Technology) One (1) each GFP generic BP filter set, these filters shall work with both wild types and mutants EXC 450-490, 498DCLP, EM 500-550. 9) One (1) each, 41002 HTQ Rhodamine/DII FL set. 10) One (1) each 31000 DAPI/Hoechst/AMCA Fl set. 11) (the following three items are manufactured by Dage MTI) One (1) each CCD-100S CCD camera system without spot mask, 1/3 inch chip interline CCD, Resolution 570 TV lines, spectral response 380 -750NM, Pixiels: 768 X485, lens mount C-Mount, black and white video output, RS-170 interface, the system includes: CCD camera head, 12 V DC power supply w/cable, instruction manual and requires BNC-BNC cable and the following accesories: >RC 100 remote control, >207633-01 1/3" coupler. 12) One each Hr-90 B/W monitor 9"-700 lines. 13) One (1) each 810080-06 6'BNC to BNC cable. 14) (the following four items are manufactured by Kinetics) One (1) each 400001-010058 Isolated work station with 30" X 36" X 1" thick vibradamped steel top, white plastic top lamination, 3" X 7" slot (1/2" radius corners) centered along table width (3" dimension centered on the 36" length), and centered 17" from the edge (7" dimension), shall include fixed shelf under table, air active table 29" height adjustable, 1" up, 1/2" down. 15) One (1) each GR-36 /9101 guard rail 36". 16) Two (2) SS-10 sliding shelf 10 x 31" white, laminate (requires guard rail). 17) One (1) each EOS electric outlet strip with GR. 18) One (1) each Micromax 1300Y interline camera system, small round camera head with C-mount electronic shuttering PI 1300*1030 CCD, grade 0, high resolution CCD (6.7*6.7 UM pixels), High QE (42%) at 520NM for FITC, GFP and FURA2, thermoelectrically cooled CCD to -10C 1 MHZ/12 BIT A/D converter, Real time RS-170 video output, standard PCI interface card, dynamic range of 4096 gray levels (12BIT), Winview software for the PC and IPLAB drivers, IPLAB spectrum software, forced air cooling shroud manufactured by Princeton Instruments. 19) (the following two items are manufactured by Scanalytics) One (1) each, IPL-MAC 3.1 spectrum image processing and analysis software for Powermac, must include: Acquisition modules for photometrics PVCAM Princeton Instruments, Hamamatsu #4742-95, Scion LG3/CG7, PCI powermac internal video, Photoshop acquire plug ins; system requirements: power PC processor, MAC OS system 73.0 or later, PCI BUS, Monitor with at least 256 colors and 800 X 600 resolution #1024X768, and RAM 32MB minimum, = / > 64MB. 20) One (1) each, multiprobe MAC Fish image analysis software, multi wavelength analysis and visualization software, this requires: IPL-MAC 2.1 spectrum software. 21) One each, #5171 000.171 micromanipulator system manufactured by Eppendorf. This system must include the following: one micromanipulator #5171, one Transjector 5246 basic, one Microscope adapter, one box of femtotips, one box of femtotips II, one cellocate 175UM sterile set, one cellocate 55UM sterile set. 22) Installation and local training. 23) One (1) Freight charge (including shipping and handling). The Standard Industrial Classification (SIC) code is 3821, size standard 500 employees. This is not a small business set aside. The request for quotation (RFQ) will be due fifteen (15) calendar days from the publication date of this synopsis. Quotations will be evaluated on conformance to the government's specifications, delivery time, price, interchange and compatibility parts with the existing equipment. The offeror must provide descriptive literature and detailed specifications as part of the quotation. The Request for Quotation (RFQ) number NHLBI-PS-98-301 will be available upon the publication of this synopsis. Requests for a copy of the RFQ must be made in writing and the request may be submitted by FAX transmission to (301)480-3345. Quotations responses must be in writing and submitted to the National Heart Lung and Blood Institute, Contracts Operations Branch, Procurement Section, 6701 Rockledge Drive, Rockledge Center Building 2, Room 6143, Bethesda, Maryland 20892-7902, Attention: Ms. Cecilia D. Morales. FAX quotations will be accepted only if dated and signed by an authorized company representative. All responsible sources may submit a quotation, which if timely received shall be considered by the agency. (0162)

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