U. S. Environmental Protection Agency, Contracts Management Division (MD-33), Attn: ORDSC, Research Triangle Park, NC 27711

66 -- ULTRA-PLUS BINARY LC GRADIENT SYSTEM SOL KK0400 POC Karen Z. Gibson (919)541-4556 E-MAIL: Click here to contact the Contracting Officer via, gibson.karen@epamail.epa.gov. The U.S. Environmental Protection Agency intends to negotiate sole source with Micro-Tech Scientific, 140 S. Wolfe Road, Sunnyvale, CA 94086 for an Ultra-Plus Binary LC Gradient System. The Contractor shall provide a Micro LC pumping system to generate binary solvent mixing that can create isocratic or gradient flow for capillary and microbore applications. The system shall have a dead volume close to zero and must produce gradient flows from a minimum of 1uL/min to at least 600 uL/min, with increments of 0.1 uL/min or less, without pulsing (as visible from data). The isocratic flow rate must deliver flow rates of 0.1 uL/min (or less) to at least 600 uL/min. The system must have a detection limit of 50 femtograms (or less), with no pulsing of the baseline. The system shall allow for rapid re-equilibration of no more than 5 min to enable fast repetitive analyses. The system must accommodate rapid switching between capillary (0.32 mm i.d.) and microbore (1.0 mm i.d.) columns. The system must include a variable wavelength UV-Vis Detector, an LC autosampler with a 0.1 uL to 100 uL variable loop, a loop injector with a minimum of a 5 uL loop, and temperature control of the column to cover the range of 10 C to 50 C. The system must be able to be directly interfaced to a Fisons Platform mass spectrometer (which has an electrospray/APCI interface), without flow splitting. The software must run on Microsoft Windows and have the potential to interface to our present Fisons Platform Electrospray MS System (which runs off a Windows environment). The software shall have the ability to acquire data, set up a series of autosampler runs, and calibrate. This data handling package shall also work for stand-alone operation of the LC system with a UV/Vis detector. The Contractor shall also install the system on-site and provide a minimum of 2-days of on-site training to EPA personnel. This system shall also include a startup kit, mass spectrometry interface kit, microbore maintenance kit, bonded columns and guard columns. EPAbelieves that Micro-Tech is the only vendor capable of satisfying the requirements stated above. No solicitation is available. All interested parties must respond within 15 days of the date of this announcement. Interested vendors shall: 1)include information on the proposed items which clearly supports the requirements outlined above and: 2) provide itemized pricing that is sufficient to make a determination that competition is feasible. Submit response to the U.S. EPA, Attn: Karen Z. Gibson (MD-33) Contracts Management Division, Research Triangle Park, NC 27711. No telephonic requests will be honored. See numbered Note 22. (0167)

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