Centers for Disease Control and Prevention (CDC), Procurement & Grants Office, Program Acquisition Branch, 255 East Paces Ferry Rd, NE, Rm 500, Atlanta, GA 30305

Q -- MOLECULAR LABORATORY TESTING ON RUBELLA VIRUS ISOLATES SOL 98A260(Q) DUE 072298 POC Susan Cleveland, Telephone: (404) 842-6715; Facsimile: (404)842-6727 The Government anticipates award of a noncompetitive contract to Teryl Frey, PhD, Department of Biology, Georgia State University, Atlanta, Georgia. Contract tasks consist of isolating rubella virus from clinical specimens from persons with serologic confirmed rubella or infants born with CRS. Nasopharyngeal and urine specimens for rubella virus isolation will be collected and rubella virus isolation will be performed using standard viral isolation procedures. An estimated 30-50 virus specimens are expected to be collected and forwarded to the contractor during a 12-month period. For rubella virus isolated from clinical specimens and rubella viral strains previously isolated: 1) The rubella virus will be amplified in cell culture and RNA will then be extracted. Polymerase Chain Reaction (PCR) will be performed and nucleotide sequences determined and 2) Nucleotide sequences will be compared with vaccine strains and wild type strains from various geographic locations. Contract term is 12 months from dateof award. Equipment required for this project includes biosafety cabinet for biocontainment Level P2, -70 C freezer space for long term specimen storage, PCR machine for DNA amplification and sequencing, pentium computer dedicated to sequence analysis. All interested parties may submit qualifications and experience information including curriculum vitae for the personnel who will be performing this work, past performance data including the size, complexity and scope of each contract or project performed which is the same or similar in nature to this project; demonstrated facilities capability to include equipment required, and laboratory certification as Level P2 containment laboratory. Evaluation criteria will include 1) Understanding the Problem; 2) Methodology and Approach; 3) Feasibility; 4) Personnel; 5) Facilities. Past performance will be used as a significant factor in determining the award of the contract. All interested parties must submit a capability statement by not later than July 22, 1998. No telephone calls regarding this announcement will be accepted. Questions may be faxed or mailed to the contracting officer. Posted 07/02/98. (0183)

Loren Data Corp. http://www.ld.com (SYN# 0066 19980707\Q-0003.SOL)