Centers for Disease Control and Prevention (CDC), Procurement & Grants Office, Contracts Management Branch, 255 E. Paces Ferry Road, NE, Room 419, Atlanta, GA 30305

B -- STUDY TO DEVELOP IMPROVED SECONDARY DNA TYPING METHODS FOR MYCOBACTERIUM TUBERCULOSIS SOL 98S282(Q) DUE 080598 POC Charlotte Flitcraft, Contract Specialist, (404) 842-6740 The Centers for Disease Control and Prevention (CDC) has a requirement for a Vendor to provide services to conduct a study to develop an improved secondary DNA typing method for Mycobacterium tuberculosis by identifying specific sites of insertion for IS6110, especially sites that are common to low copy number isolates, and characterize "hot spots" for insertion. The Vendor will be required to develop DNA probes to detect specific insertions and evaluate the probes with low copy number isolates to determine the utility of the method as a secondary typing tool. CDC will furnish the following materials to the Vendor: 1) four isolates of M. tuberculosis; 2) 100 Isolates or DNA Samples; 3) Molecular Weight Standard; 4) IS6110 Probe; and 5) the protocol for standard DNA fingerprinting using IS6110. Potential Vendors shall meet the following minimum qualifications: 1) Two years experience in DNA fingerprinting of M. tuberculosis and related molecular biology techniques; 2) have existing equipment for image scanning and analysis using the BioImage Whole Band Analysis software package; 3) one year prior experience in safe handling of M. tuberculosis; and 4) approved Biosafety Level 3 laboratories (as specifically detailed in "Biosafety in Microbiological and Biomedical Laboratories", HHS Publication No. 93-8395) for conducting the study. The period of performance will be for 12 months. A copy of the Request for Quotations (RFQ) can be obtained by submitting a written request to Charlotte L. Flitcraft. This request may be submitted by facsimile at (404) 842-6740 or by e-mail a caf5@cdc.gov., by August 4, 1998, RFQ Number 98S282(Q). No telephone requests will be accepted. The RFQ will be released on or about August 5, 1998. The SIC is 8733, $5M. See Numbered Note 1. Posted 07/17/98 (I-SN225103). (0198)

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