Contracting Officer (90C), DVA Medical Center, 700 South 19th Street, Birmingham, AL 35233

66 -- CONFOCAL MICROSCOPE SOL RFP 521-075-98(EP) DUE 091598 POC Edward G. Phillips, (205) 933-4492 This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in Subpart 12.6, as supplemental with additional information included in this notice. This announcement constitutes the only solicitation; proposals are being requested and an written solicitation will not be issued. Solicitation NO. RFP 521-075-98 (EP). This solicitation document and incorporated provisions and clauses are those in effect through Federal Acquisition Circular 97-06. The SIC for this solicitation is 3826. Item description: Contractor shall provide to the VA Medical Center Research and Development Service, Birmingham, Alabama, the Leica Inverted Confocal Microscope TCS NT SP UV, 3 spectrophotometer Imaging Detectors and Transmitted Detector. Requirements: Scan head must use a spectrophotometer for each photomultiplier rather than filters; The detection range must be 380-800nm; The microscope must have "Design a Filter" capability so that spectral head provides for complete experimental freedom in selecting the wavelengths for detection. Detection must be capable of being set in intervals of 1nm. Minimum slit width must be 5nm; Wavelength scans to determine the spectral emission of any fluorochrome or autofluorescence must have a minimum bandwidth of 5nm that may be stepped in increments of 1nm; Pixel resolution of the spectrophotometer must be capable of better spectral steepness and transparency than filter combinations. It must be capable of being set to the real emission spectra of dyes. It must produce less bleed through in multi-color applications; Must be capable of multi-channel recording of emission spectra. In addition to the UV laser, there must be a 60 mW Ar Laser 488, 514, a 25mW Kr Laser 568, and a 12 mW HeNe Laser 633. There must be provision to add 4th visible laser- HeCd 440;The microscope must have true confocal design with both an illumination pinhole for the visible lasers and a separate pinhole for the UV laser. Also must have a continuously variable detection pinhole;The pixel registration must be guaranteed in XYZ between all detection channels;There must be a single mirror design to maintain the telecentric illumination of the objectives; Geometric distortion of displayed image must be < 1%; Scan speeds must be to 3 frames/sec at 512x512 pixels, 10 frames /sec at 64x64 pixels, 1900 lines/sec; Scan format must be selectable from 64x64 pixels to 2048x2048 pixels; Must have scan modes XY, XZ, XT, XYT, XZT, XYZ and XYZT; There must be six selectable scan speeds with uni-directional or bi-directional scanning; Laser beam must blank during fly back; Optical Transfer Efficiency must be = 92% at 450 nm; Beam path must be unfolded with one first surface scan mirror and two correction lenses in detection path; Beam path in the scan head must be approximately 20 cm; Resolution must be guaranteed 180 nm XY, 350 nm XZ (488nm illumination in reflection mode); Galvonometer-driven Z stage must allow for minimum step width of 10nm, reproducibility must be equal to 40 nm, must provide foron-line XZ scanning (>10 frames/sec at 64x64 and 3 frames/sec at 512x512), rotatable 360 degrees; There must be a panel box control of scanning parameters; Image capture must have 8 Bit dynamic range when using the Extended Dynamic range option. File format free upgrade must be available in 16 Bits during FY99, possibly as early as December software release; The software must have straightforward interface designed for multi-user and custom applications; The software must have multiple display formats from 256x256 pixels to 1024x1024 pixels; Software must be capable of displaying up to four channels at once: Display format must be independent of acquisition format; Software must be capable of selecting gallery modes, single modes and overlays; All scanning functions (gain, offset, focus, pinhole, zoom & phase adjustment) must be adjustable with Panel Box; Software must include programmable time scan functions with input and output triggers, XT, XY over time or XYZ over time, and programmable scan routines; Software must have quantification functions: intensity, length and area measurements, ratioing of images, static and time measurements, XT series quantification and topographical measurements. Data formatted to be capable of exporting to Excel format; Physiology, FRAP, Bio-mapping and custom software must be available; Software must be capable of 3D viewing and rotations with six algorithms for 3D rendering (Extended focus, Maximum projection, No Light, Lighting with adjustable angles, Solid Rendering and SFP);Software must be capable of orthogonal and diagonal slicing of 3D data sets; Software must be capable of automated sequential scanning routines; Software must be capable of spot bleaching routine; All data must be stored in universal TIFF file or AVI file (movie functions); There must be an On Line Help and Tutorial; C-3 System Components: System shall consist of the following components:Leica DMIRBE Inverted Research Microscope UV, Basic Stand for Fluorescence Motorized coarse and fine focus Motorized 6 place focusing nosepiece 7mm of Focus Travel Infinity corrected Optical System with built-in Bertrand lens, Integrated 5-12V/100 W power supply for Transmitted Light Illuminator, Integrated LCD Display and RS-232 Interface, UV Filtersystem A Binocular Observation, Tube BSA 20/45 Eyepiece Pair, 10X/20 with Eyecups, 3 Plate X-Y Manual Stage,Mercury Illuminator 105Z, 550W HBO Incident Light Fluorescence Equipment Filter System, I 3 Filter System, N 2.1 Turret Condenser S1 / 0, 9NA Halogen Illuminator 105, 12V 100W Halogen Immersion, Oil Dust Cover, PL APO 10x/0.40 Imm UV, PL APO 20x/0.70 Imm, PL APO 40x/1.25 -- 0.75 Oil, PL APO 63x/1.32 Oil *UV; Accessories for Nomarksi and Transmitted Detector -- ICT Basic Outfit Inverted; Consisting of: Polarizer ICT disk, coded ICT Prism C, Analyzer IC, IC Prism D, IC Prism E, ICT Condenser Prism K4, ICT Condenser Prism K5, Transmitted Light Detector DMIR, Galvanometer driven Z-stage including rot. stage insert, Z motion precisely controlled by galvanometer reproducibility better than 40nm, Leica Power 3D Software Package Stereo, Rotations, 3D & Slicing, Raytracing, Volume rend.,Simulated Fluorescence Process, Leica TCS SP Three Channel Scan Head (VIS & UV), Cardanic Single-Mirror Scanner, Linearity better than 99%, Miniaturized Optical Path for Long-term stability, Optical Transfer Efficiency 0.92, 1 High Sensitivity Side Entry Photomultiplier R4457 P, True Confocal Optical Path with One Excitation Pinhole and One Computer-Controlled Detector Pinhole (Variable) for exact cc-localization of Multi-Labeled Specimens, Scanner Fully Automated & Computer-Controlled, Spectrophotometer Detector SP, 3 Reflected/Fluorescence Channels, Scanner switchable between DMR Upright Microscope and DMIRB Inverted Microscope, 5 Minutes Conversion Time; No Re-Calibration required, One Anti-Vibration Microscope Table & one Computer Table, Photomultiplier R 3896 (Qty 2) very high efficiency, high red and blue sensitive, recommended for extremely low fluorescence yield measurement, includes housing, mount, high voltage supply and preamplifier, Leica TCS NT Complete Electronics, Workstation and 21" Dual Monitor 1280 x 1024 Display, Multitasking High Performance Windows NT Pentium Workstation Simultaneous Acquisition of 4 Data Channels, TCS NT Scan Driver Electronics & System Controller for up to 4 channels, Pentium 6 / 200MHz, 128MByte Tower Crate, 2.1 GB SCSI Hard Disk, 3.5" Floppy Drive, CD 4xspin, High Performance Frame Grabber, 3 Com Ethernet board, SCSI Adaptec Controller, 2 ea. 21" Image Monitor, True Color, total 2560 x 1024 Pi Panel-Box with 7 Digital Potentiometers for fast, online adjustment of scanning parameters, MS Keyboard, Mouse, Data Modem internal 28.800 bps, MS-WINDOWS NT 4.0, Excel NT 7.0, Leica Power Scan Software for Scan Control, Display & 2DTCP/IP Software Online Software & Hardware Manuals; Complete 3 Laser Configuration with AOTF for TCS NT, HeNe Laser (633nm wavelength), Argon Laser (488nm wavelength), Kr Laser (568nm wavelength) Merge Module, couplings and Acousto Optical Tuneable Filter AOTF; C-4 Training: The contractor shall provide training sessions to include 2 training sessions on 2 consecutive days for all personnel in Research Service who will use instrument. Each session will include "hands on" instruction, literature, training manuals, and reference guides; C-5 Manuals: The contractor shall provide a minimum of two (2) service manuals, and two (2) operating manuals to the Contracting Officer's Technical Representative (COTR); C: Installation: The contractor is responsible for the installation and set-up of the Leica Inverted Confocal Microscope TCS NT SP UV, 3 spectrophotometer Imaging Detectors and Transmitted Detector. The provisions at FAR 52.212-1, Instructions to Offerers Commercial applies to this solicitation. The Clause at FAR 52.212-3 Offeror Representations and Certifications-Commercial items, applies to this solicitation. Offeror must submit with proposal a completed Representations and Certifications. The clause at FAR 52.212-4, Contract Terms and Conditions-Commercial Items, applies to this solicitation. The clause at FAR Part 52.212-5. Contract Terms and Conditions Required to Implement Statues or Executive Orders-Commercial items, applies to this solicitation. The VA intends to solicit and negotiate with only (1) one source under the authority of FAR 6.302 for a commercial item as stated above. Interested parties may indentify their interest and capability to respond to the requirements or submit proposals. This notice of intent is not a request for competitive proposal. However, all proposals received within fifteen days after date of publication of this synopsis will be considered by the Government. A determination by the Government not to compete this proposal contract based on responses to this notice is solely within the discretion of the Government. Information received will normally be considered solely for the purpose of determining whether to conduct a competitive procurement. VAMC Birmingham intents to negotiate with: Leica Microsystems, Inc., 410 Eaglevie Blvd., Suite 107, Exton, PA 19341 (610) 321-0460. Posted 08/31/98 (W-SN243808). (0243)

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