National Cancer Institute, Research Contracts Branch, PSAS, 6120 Executive Blvd, EPS/Room 638, Bethesda, MD 20892-7227

66 -- SMART SYSTEM PACKAGE SOL RFQ80213(NR) DUE 092198 POC Marsha Gorham, Purchasing Agent, (301)402-4509, & Todd Cole, Contracting Officer The National Cancer Institute (NCI) plans to procure a Smart System Package from Amersham Pharmacia Biotech, Inc., 33148 Treasury Center, Chicago, IL 60694. The NCI has a need for rapid purification of proteins and for analyzing protein to protein interactions in micro-amounts. The system will be specifically used for the purification and characterization of mutant RNAPs. The need to isolate enzymes from different mutant stains can be accomplished, thereby reducing time and costs, if micropurifications can be performed. In addition, the researcher requires such a system in order to perform protein to protein interactions with a minimal amount of protein and ensure maximum signal-to-noise ratio. The basic system shall be comprised of the following: a Microprecision Pump, a Variable Volume Dynamic Gradient Mixer, a Microseparation Unit, an Injector, a Micropeak Monitor, a Microfraction Fraction Collector, an Internal Cooling Device, and appropriate Control and Evaluation Software. The system shall also have the following specific features: 1) The system shall be a micropreparative system designed to isolate a microgram and less amounts of biomolecules from a wide range of starting volumes (5.0 ml to 50 ml); 2) The system shall utilize liquid chromatography column technology precision columns packed with a broad selection of media; 3) It shall have precision columns that have internal diameters from 1.6 to 3/2 mm and column lengths from 2.0 to 30.0 cm; 4) The system shall achieve pulse-free gradient or isocratic separations; 5) The system shall also prevent the formation of salt crystals; and 6) The microprecision pump shall have a flow range of 10 -- 2000 ml with a pressure limit of 25 mpa. 7) The system shall have special device to automatically connects columns with minimal dead' volume; 8) The system shall have fibre optics for sensitive UV monitoring of microliters' volume, 9) The system shall have accurately and reproducibly collect fractions as small as 5 microliters; and 10) The system shall have an internal cooling device to cool to 4(C throughout the integrated flow path. The Smart System Package from Amersham Pharmacia Biotech, Inc is the only system known to NCI that can meet the requirements herein. If any interested party believes it can meet the above requirement with an equal system, it may submit a statement of capabilities. The statement of capabilities and any other information furnished must be in writing and must contain material in sufficient detail to allow NCI to determine if the party can meet this requirement. Capability statements must be received in the contracting office by 3:00 p.m. EST, on September 21, 1998. If you have any questions, please contact Marsha Gorham, Purchasing Agent on (301) 402-4509. A determination by the Government not to compete this proposed requirement based upon responses to this notice is solely within the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. Posted 09/03/98 (W-SN245324). (0246)

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