National Cancer Institute, Research Contracts Branch, PSAS, 6120 Executive Blvd, EPS/Room 638, Bethesda, MD 20892-7227

66 -- UPGRADE AN ATTOFLUOR IMAGING SYSTEM SOL RFQ80244(NR) DUE 092598 POC Marsha Gorham, Purchasing Agent, (301)402-4509 & Todd Cole, Contracting Officer The National Institute of Neurological Disorders and Stroke (NINDS) plans to upgrade an existing Attofluor Imaging System, including a Confocal Attachment and a Cooled Charge-Coupled Device Camera (CCD Camera) from Carl Zeiss, Inc..One Zeiss Drive, Thornwood, NY 10594. The upgrade shall be assembled on an existing Attofluor Ratiovision System and it will be used for optical imaging of cellular and subcellular signals to allow NINDS to follow changes in second messenger signals with greatly improved spatio-temporal resolution virtually on-line. It will also allow NINDS to fully explore and understand such important biomedical issues as physiological regulation of developmental changes occurring in the embryonic CNS. The system shall: 1) be capable of realtime direct confocal fluorescence viewing and documentation at three wavelengths with motorized optical sectioning and image acquisition software; 2) calibrated excitation and/or emission ratioing with simultaneous background subtraction, shading correction and exclusion with up to 99 Regions of Interest (ROI) plus post-experimental analysis from stored images; 3) windows software for experimental flexibility; 4) simultaneous or sequential multi-dye capability; 5). integrated att data analysis software (AttoGraph) which can also be run on any stand alone PC; 6) integrated with Atto Graph is Atto View which allow flexible image viewing from experimental data sets; 7) high quality 12 bit imaging with a cooled CCD camera (Orca-100/PC) allowing low-level fluorescence light acquisition; 8) c-apochromat 63x1.2 water corrected objective for viewing optical sections in multilayer tissue slice preparation. Carl Zeiss Inc, is the only source known to NINDS that offers an upgrade to the existing Attofluor Ratiovision System. However, if any interested party believes it can meet the above requirements, it may submit a statement of capabilities. The capability statement and any other information furnished must be in writing and must contain material in sufficient detailto allow NINDS to determine if the party can meet the mentioned requirements. Capability statements must be received in the contracting office by 3:00 p. m. EST, on September 25,1998. If your have any questions, please contact Marsha Gorham, Purchasing Agent, on (301)402-4509. A determination by the Government not to compete this proposed contract based upon responses to this notice is solely within the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. No collect calls will be accepted. Posted 09/08/98 (W-SN247017). (0251)

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