National Institutes of Health, Office of Procurement Management, 6011 Executive Blvd., Rockville, Maryland 20892-7260

66 -- GENECHIP ANALYSIS SYSTEM SOL 263-98-P-(BP)-0047 DUE 092998 POC Ms. Dawn Rabunsky, Contract Specialist, 301/402-3068 66 -- THIS SYNOPSIS/SOLICITATION FOR COMMERCIAL ITEMS DESCRIPTION, PREPARED IN ACCORDANCE WITH FORMAT IN FAR 12.6, AS SUPPLEMENTED WITH ADDITIONAL INFORMATION INCLUDED IN THIS NOTICE. THIS ANNOUNCEMENT CONSTITUTES THE ONLY SOLICITATION, WRITTEN RESPONSES ARE BEING REQUESTED AND A WRITTEN SOLICITATION WILL NOT BE ISSUED. THE GOVERNMENT INTENDS TO AWARD THE CONTRACT TO AFFYMETRIX,INC. WITH IN ACCORDANCE TO FAR 6.302-1, CITATIONS 10 U.S.C.2304(C)(1) OR 41 U.S.C.253(C)91). THE SOLICITATION NO. 263-98-P(BP)-0047, IS ISSUED AS A COMMERCIAL ITEM ACQUISITION. THE SOLICITATION/CONTRACT WILL INCLUDE ALL APPLICABLE PROVISIONS AND CLAUSES IN EFFECT THROUGH FAR FAC 97-6. INDEPENDENTLY AND NOT AS AN AGENT OF THE GOVERNMENT, THE CONTRACTOR SHALL FURNISH AND INSTALL AND PROVIDE TRAINING ON A GENECHIP ANALYSIS SYSTEM INSTRUMENT CAPABLE OF ACCOMMODATING MULTIPLE USERS AND HAVE THE CAPABILITY FOR FUTURE UPGRADING AS NEED ARISES. IT MUST BE A VERSATILE SYSTEM OPTIMIZED FOR EASE OF USE AND MAINTENANCE. IT MUST INCLUDE BUT NOT BE LIMITED TO THE FOLLOWING SALIENT CHARACTERISTICS: 1) The GeneChip Hu6800 set probe arrays quantitatively detect expression levels of approximately 6800 full length human genes. 2) The GeneChip Mu6500 set probe arrays quantitatively detect expression levels of approximately 6500 gene and EST sequences. 3) Each Gene of EST is analyzed with approximately 20 pairs of specific, unique 25mer oligonucleotide probes. 4) Pairs of Perfect and single-based mismatched probes are synthesized on adjacent areas on arrays. Data analysis requires this probe pairing strategy so that nonspecific hybridization and background signal can be identified and subtracted. 5) Over 65,000 difference oligonucleotide probe sequences are synthesized in-situ on a 12.8 mm x 12.8mm probe array. 6) Each probe sequence is located in a specific area on the probe array called a probe cell measuring approximately 50 microns by 50 microns. 7) Each probe cell contain approximately 10 copies of a given probe. 8) Sensivity of detection is from a few copies to several hundred copies per cell. 9) Each array contains probe complementary to the bacteria genes biob, bioc,, biod, and cre, which are used as normalization controls. 10) Each array in either set can detect >1300 specific genes or ESR'S. 11) Each Array is packed in a self-contained disposable cartridge which acts as a reaction chamber. 12) Prior to scanning, the Genechip probe array is stained and washed using an automated protocol and instrument. 13) The fluidics station then performs the buffer introductions, mixing, washing, and other steps automatically. 14) Each fluidics station can independently process four differenct probe arrays in parallel. 15) Upon completion, a message displayed on the PC screen and lights on fluidics station indicate that the probe arrays are ready for scanning. 16) The scanner user an argon-ion laser to excite fluorescense molecules incorporated into the target hybridized to the probe array. 17) The excitation wavelength is 488nm and the laser spot sizeis 4 microns maximum full-width at half maximum. Pixel size is adjustable to 3,6,9,12, and 24 microns. 18) Thermally compensated optics and low electronic noise enable high scanning sensivity . The scanner operates at 100-120 V, 6A 50/60Hz, and THD of 10%. 19) The scanner dimensions are 42 cm height, 66cm deep and 78 cm wide. The total single pass scan time for a 12.8 mm by 12.8mm GeneChip Probe Array is maximum of 6 minutes. 20) Emitted light at 570 nm is collected to determine the amount of hybridized target at each location on the probe array. 21) The GeneChip software controls the GeneChip Fluidics Station 400 and hewlett Packard Gene Array Scanner. Information about the experiments is collected in a dialog box that creates the experiment information file (*.exp) with a unique name. 22) During Scanning an image of the probe array is displayed. In real time while pixel-specific intensity information is automatically stored in a raw file (the *.dat file). 23) The scanned probe array image is analyzed andaligning a grid and calculating average intensities for each probe cell and assigning each cell an x,y coordnate position. 24) The output of this analysis (the *.cel file) can be used to reanalyzed data with different expression algorithm parameters are user adjustable and defaults are provided. 25) Many expression algorithm parameters are user adjustable and defaults are provided. 26) The output (the *chp file) contains absolute analysis and may contain the comparision analysis. 27) The absolute analysis determines which transcripts are present or absent in a sample and the comparison analysis determines which transcripts are increased or decreased between the samples. 28) Training should take place at the Government's site. The contractor shall provide the four attentees with all material, text material, and course outline necessary for the specific training. The contractor shall provide a minimum of three working days of on-site training. Training shall be eight hours per day and performed between the hours of 9:00am and 5:30pm. Training shall include be not be limited to proper operation of the instrument, cleaning, instructomaintenance, minor adjustments, and troubleshooting. The instructor shall be experienced with the GeneChip instrument and have a thorough understanding of the theory of operation, the underlying physical principles, and the practical sage application of the instrument. In particular, the instructor shall have expert knowledge of the software used to operate the microscope and provide assistance in customizing the instrument to facilitate its operation. The contractor shall work constructively with the Government to optimize performance of the geneChip analysis system and resolve unanticipated problems. THE REQUIRED DELIVERY DATE IS DECEMBER 21, 1998. THE INSTRUMENT SHALL BE DELIVERED FOB DESTINATION, TO THE FOLLOWING ADDRESS: NATIONAL iNSTITUTES OF HEALTH, NIDA 5500 NATHAN SHOCK DRIVE, ROOM 329 BALTIMORE, MARYLAND 21224 EVALUATION CRITERIA: DESCRIPTIVE LITERATURE MUST BE INCLUDED WITHTHE OFFEROR'S PROPOSAL. TECHNICAL. THE CONTRACTOR MUST DEMONSTRATE THAT THE INSTRUMENT MEETS ALL THE SPECIFICATIONS IDENTIFIED HEREIN. PAST PERFORMANCE: THE CONTRACTOR SHALL PROVIDE A LIST OF AT LEAST THREE PREVIOUS GOVERNMENT CONTRACTS FOR SAME OR SIMILAR INSTRUMENT. THE CONTRACTOR SHALL INCLUDE: CONTRACT NUMBER, CONTRACT AMOUNT, CONTRACT TYPE, INSTITUTION, CONTACT PERSON, CONTACT PERSON PHONE NUMBER AND ADDRESS. COST. THE CONTRACTOR MUST PROVIDE AN INTERNAL PRICE LIST OR CATALOG THAT CONTAINS THE PRICING FOR THE GENECHIP INSTRUMENT DESCRIBED HEREIN REPRESENTATION AND CERTIFICATION. THE CONTRACTOR MUST COMPLETE THE ATTACHED REPRESENTATIONS AND CERTIFICATIONS. CLOSING DATE: ALL BIDS MUST BE RECEIVED BY 11:00 PM (LOCAL TIME), SEPTEMBER 29, 1998. NOTE #1 Posted 09/14/98 (W-SN249491). (0257)

Loren Data Corp. http://www.ld.com (SYN# 0330 19980916\66-0009.SOL)