DHHS/PHS/FDA/Office Of Facilities, Acquisition, And Central Services, Division of Contracts and Program Management, HFA-512(COB), 5600 Fishers Lane, Rockville, MD 20857

A -- MOLECULAR MECHANISMS OF DEVELOPMENTAL TOXICITY AND ABNORMALITIES SOL 267805-00-99 DUE 102798 POC Trudy Dartouzos, Contract Specialist, ph: 301/827-7165; fax: 301/827-7103 Note: This announcement is for information purposes only and will be made on a sole source basis by COB 10/27/98. This award is a planned solicitation under the simplified acquisition process. The Food and Drug Administration [FDA], Center for Devices and Radiological Health [CDRH], intends to award a purchase order to Pathology Associates International based on the Scope of Work for technical services to complete the following tasks: 1] Perform gross and histologic examination of fetal/embryonic tissue to assay segmental and other defects, 2] Analyze embryonic tissues for qualitative and quantitative changes in genetic expression and identify unique proteins that are expressed in response to physical and chemical exposure using gel electrophoresis technicques, immunochemical/immunohistochemical methodologies, 3] Perform assays to identify alterations in the proliferation cells with the presomitic mesoderm and other issues of the developing embryo following in vivo and in vitro hypertermia treatment. These assays will incorporate immunocytochemical techniques such as detection of prolifering cell nuclear antigen and/or in situ hybridization for histone mRNA, 4] Analyze embryonic tissues for the specific DNA endonuclease activity that is accelerated during programmed cell death using some of the following techniques: endonuclease activation, gel electrophoresis for DNA fragmentation, in situ hybridization and quantitative RT-PCR, and 5] Analyze embryonic tissues for identification of unique gene expression following hyperthermia using termo-stable DNA polymerase and specific primers for a specific sequellnce or gene of interest [heat shock genes/proteins; homeobox genes/proteins, etc.]. Period of Performance: date of award to one year. All equipment, research materials and facilities will be furnished by the Government at the Center for Devices and Radiological Health Laboratories. Posted 10/07/98 (W-SN259858). (0280)

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