National Institutes of Health, National Heart, Lung, and Blood Institute, Contracts Operations Branch, 6701 Rockledge Drive, Room 6100, MSC 7902, Bethesda, MD 20892

A -- MAMMALIAN GENOTYPING SERVICE SOL N01-HV-48141 DUE 113098 POC Sharon M. Kraft, (301) 435-0340 E-MAIL: Click here to contact the contracting officer via, sk40F@nih.gov. The National Heart, Lung, and Blood Institute intends to negotiate, on a non-competitive basis, with the Marshfield Medical Research & Education Foundation for a seven year extension of contract no. N01-HV-48141 for the NHLBI Mammalian Genotyping Service. Authority: 41 U.S.C. 253(c)(1) as set forth in FAR 6.302-1. In 1994, the Marshfield Medical Research & Education Foundation established a large-scale, high through-put genotyping service as a cost-effective means to facilitate the identification of genes involved in cardiovascular, pulmonary, and hematologic diseases. The NHLBI Mammalian Genotyping Service utilizes scanning fluorescent densitometry to run multiplex polymerase chain reaction samples using custom automated methods and robotics. The Service does not isolate genomic DNA from tissues or cells, provide long term storage of DNA, or offer data analysis. The Service currently accepts samples from human, mouse, and rat studies. Genotyping projects are submitted to the Service, wherein areview panel of scientific experts, established and coordinated by the Contractor, assesses and recommends projects for access to the Service. Projects outside of NHLBI programmatic relevance are considered for genotyping; however, meritorious projects with NHLBI programmatic relevance receive extra consideration. Genotypic data are provided back to users of this service as FTP files. The Service ensures confidentiality of human subjects and genotyping data. The Service has focused on increased production capacity, while reducing the cost per genotype. The current capacity is 4,000,000 genotypes per year with a total cost of approximately $0.50 per genotype for standard human genotyping at a spacing of 10 cM. The average error rate for the Service at this through-put rate is less than 1 percent. It is expected that the capacity will continue to increase and the costs will continue to drop, with the aim of 13,000,000 genotypes per year at a cost of under $0.30 per standard human genotype by the end of seven years. The Service has been responsible for continuously incorporating newly developed technologies, methodologies, and their refinements into the existing structure to improve genotyping quality, to decrease costs, and to increase the production of genotypes. The intention of extending the contract with Marshfield Medical Research & Education Foundation is on the basis that no other organization is capable of producing this number of genotypes at this quality, efficiency, and cost without great duplication of time and cost to the Government. The incumbent contractor has the unique capability to provide these required services to the NHLBI for continuation of this project. This is not a Request For Proposals. See Note 22. Posted 10/15/98 (W-SN262491). (0288)

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