National Cancer Institute, Research Contracts Branch, PSAS, 6120 Executive Blvd, EPS/Room 638, Bethesda, MD 20892-7227

66 -- LIGHTCYCLER LC32 SOL RFQ90022-NS DUE 121598 POC Patricia Haun, (301) 402-4509 The National Cancer Institute (NCI), Division of Clinical Sciences proposes to procure a Lightcylcer LC32 from Appropriate Technical Resources Inc.(ATR), P.O. Box 460, Laurel, MD 20725. The Lightcycler LC32, which is manufactured by Idaho Technology with distribution by ATR, will allow NCI researchers to: determine the initial copy of a number of unknown samples; quantify the total DNA or RNA; design and use economical probes for identification of specific DNA mutations and sequence polymorphisms; use reagents in quantities ten times less than heat block thermal cycler machines; and use competitive PCR and reannealing kinetics, as well as DNA melting curve analysis. The basic system shall have the following characteristics: 1) The machine shall integrate a 32 sample micro-volume fluorimeter with an air driven ultra-rapid thermal cycler to deliver quantitative real time analysis of DNA or RNA amplification; 2) The system shall allow continuous monitoring of the reaction and perform 30-40 cycles of amplification in less than 10 minutes on volumes of less than 10ul (This eliminates the need for separate amplification and product analysis); 3) the system's sample cuvettes shall have a thin glass capillary design (for large surface area to volume ratio and heat conductivity) and provide elimination of a sample block by airflows and allow for faster temperature changes; 4) the cuvettes shall also allow for fluorescence acquisition via a light-piping tip; 5) the machine shall also enable gene quantification to the single copy level within 10 minutes; 6) the instrumentation shall utilize a broad spectrum of fluorescent emissions and com with a three color optics modules or better; 7) the machine shall also provide nonhydrolytic probes for easier design and optimization by the researcher, and 8) the system shall use monitoring fluorescence upon binding utilizing an approach that allows product differentiation by analysis of product melting curves. Based on the above, Appropriate Technical Resources is the only source known to NCI that can provide a light cycler that achieves real-time thermal cycle monitoring followed by DNA melting kinetics. This notice of intent is not a request for competitive proposals. However, if any interested party believes it can perform the above requirement, it may submit a statement of capabilities. The statement of capabilities and any other information furnished must be in writing and must contain material in sufficient detail to allow NCI to determine if the party can perform this requirement. Capability statements must be received in the contracting office by 3:00 p.m. EST, on December 15, 1998. If you have any questions, please contact Patricia Haun, Purchasing Agent on (301) 402-4509. A determination by the Government not to compete this proposed requirement based upon responses to this notice is solely within the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. Posted 11/24/98 (W-SN274564). (0328)

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