Naval Research Laboratory, Code 3220, 4555 Overlook Ave. S.W., Washington, D.C. 20375-5326

66 -- ADVANCED LASER SCANNING CONFOCAL MICROSCOPE SOL N00173-99-R-KK01 POC Kevin King, Contract Specialist, Code 3220.KK, (202) 767-1495, Wayne A. Carrington, Contracting Officer WEB: click here, http://heron.nrl.navy.mil/contracts/home.htm. 66-The Naval Research Laboratory (NRL) has a requirement for an advanced laser scanning confocal microscope system. The system is required to image in one or more z-axis planes to allow three dimensional reconstruction of thick (> 50 micron) samples. This system is required to have ultraviolet excitation (or be capable of being upgraded to have ultraviolet excitation), inverted imaging, and be capable of performing digital reconstructions. The instrument must also be capable of imaging a common range of visible spectrum flurophores, capable of ultraviolet excitation of fluorophores in confocal imaging mode, capable of acquiring simultaineous confocal images of at least two fluorophores and allowing for digital ratiometric combination of these images, and be capable of confocal imaging in both reflectance and fluorescence modes. The instrument should have maximum adjustability toward the true spectra of a wide variety of existing and newly developed fluorophores in the ultraviolet and visible spectrum, and be capable of being upgraded to two-photon (picosecond) excitation using infrared pulses for nondestructive imaging of biological samples. This system must be compatible with an existing Leica inverted microscope. The equipment shall consist of the confocal microscope, objective lens, spectral confocal scanner system, pin hole optics with a 3 laser configuration and related computer controlled hardware and software. It is NRL's intent to make an award from this solicitation using the commercial item procedures of FAR Part 12. NRL uses Electronic Commerce (EC) to issue RFPs and amendments to RFPs. This solicitation and any amendments to it will be available via the internet at the URL address shown below. Paper copies of the RFP will not be provided. 18. LINKURL: http://heron.nrl.navy.mil/contracts/home.htm 19. Click Here 20. Cite: (W-339 SN148438) Posted 12/03/98 (W-SN276841). (0337)

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