USDA, APHIS, Field Servicing Office, Butler Square West, 5th Floor, 100 North 6th St., Minneapolis, MN 55403

99 -- LIQUID TRIMEDLURE SOL 38-M-APHIS-99 DUE 121898 POC Jeanne V. Lewis (612) 370-2109 This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in FAR Subpart 12.6, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation;proposals are being requested and a written solicitation will not be issued. This solicitation document and incorporated provisions and clauses are those in effect through Federal Acquisition Circular 97-06. The Standard Industrial Classification is 8999; This is a 100% small business setaside. The USDA, APHIS, Mpls-ABS has a requirement for 280 kilograms of liquid trimedlure to be delivered in 40 drums. LIQUID TRIMEDLURE SPECIFICATIONS: TRIMEDLURE CONCENTRATE SAMPLING & TESTING PROCEDURES: A. General: USDA will conduct chemical analysis of each lot of Trimedlure concentrate for purity and isomer composition prior to it's incorporation into dispensers. B. Sampling for Lot Acceptance: For purposes of sampling, all Trimedlure concentrate manufactured from a single production run is defined as a lot and the coded designation of a lot as the lot number. C. Test Procedures: Infrared and gas chromatography analysis will be used for identity of fruit fly attractant. The sample submitted to the laboratory shall be well mixed. Evident lack of homogeneity shall be cause for rejection. 1. Identity by Infrared Spectroscopy. A solution shall be made by dissolving 10 to 12 mg of sample in 1.0 ml of infrared grade carbon disulfide. After mixing, solution shall be placed in a 0.5 mm path-length cell and scanned from 4,000-to 650 cm with a similar cell filled with carbon disulfide in the reference beam. The spectrum of the sample shall be qualitatively the same as the spectrum of authentic Trimedlure. The relative intensities of the peaks shall be the same as those of the reference spectrum (attached as Figure 1) and have no extraneous absorbencies. 2. Purity by Gas Chromatography. A solution shall be prepared by dissolving a weighed quantity of sample in hexane to give a known concentration close to 1.0 ug/ul. A 2 ul aliquot of this solution shall be injected onto a wide-bore (0.75 mm i.d. x 30 m long) SPB-1, non-polar, capillary column programmed at 100 degrees C for 2 minutes and then 10 degrees/minute to 250 degrees C where it was held for 6 minutes. Under these conditions, Trimedlure elutes as a pair of unsolved peaks about 5.9 and 6.0 minutes; dehydrochlorinated precursor elutes about 3.6 minutes. The purity of the sample shall be computed by first summing the areas of all of the peaks (other than those attributable to background) that appear in the chromatogram beyond the solvent front (usually beyond 2.5 minutes); the percent Trimedlure shall then be obtained by dividing the sum of the areas of the pair of Trimedlure peaks by the total area of all sample peaks and multiply by 100. The amount of dehydrochlorinated Trimedlure shall be obtained by dividing the area of its peak by the total area of sample peaks and multiplying by 100. 3. Isomer Composition by Gas Chromatography. The isomer composition of Trimedlure shall be measured by capillary column, gas chromatography on a 60-m long x 0.25 mm i.d. fused-silica column coated with Durawax DX4, Carbowax 20M, or other equivalently polar stationary phase (helium or hydrogen carrier gas at roughly 1 ml/min., injection port and detector temperatures of 225 and 275 degrees C, respectively); 2 ul of the solution prepared in C.2 shall be injected onto the column set at 100 degrees C for 1 minute, then programmed at 1-5 degrees C/min. to 185 degrees C where it is held for 15 minutes. The resulting chromatogram should show the separation as indicated in Figure 2 (attached). The composition shall be determined by first summing the area for all eight isomer peaks. The amount of cis- Trimedlure shall be obtained by multiplying the total area of the four cis-isomers times percent purity (measured in C.2) and dividing by the total area of all eight isomer peaks. If the second cis peak at 20.52 minutes (Figure 2) is unresolved from the trans B2 peak at 20.21 min., the area of the cis peak at 22.88 minutes shall be taken also as the area of the unresolved cis peak; the B2 area shall be corrected accordingly. The B2 and C isomer contents shall be determined by multiplying the area of the B2 (or C) peak times the percent purity and dividing by the total area of all eight isomer peaks. PERFORMANCE SPECIFICATIONS -- A. Chemical Assay: USDA will conduct chemical analysis of each production lot of trimedlure for isomer content purity, refractive index, infrared spectum. B. Bioassay : In addition to chemical analysis, the Government reserves the option to conduct bioassay field tests to determine the effectiveness of the product for the intended purpose. Failure of the product to meet bioassay requirements will be cause for rejection. Only Trimedlure which has been tested and approved by the USDA for program use will be used as a control. FIRST ARTICLE APPROVAL -- GOVERNMENT TESTING: (a) The Contractorshall deliver 0.5 kilograms of Item 1 within 45 calendar days from the date of this contract to USDA, APHIS, IS, 54 East Fronton Street, Brownsville, TX 78520 for first article tests. The shipping documentation shall contain this contract number and the Lot/Item identification. The characteristics that the first article must meet and the testing requirements.(b) Within 60 calendar days after the Government receives the first article, the Contracting Officer shall notify the Contractor, in writing, of the conditional approval, approval, or disapproval of the first article. SPECIAL PACKING AND MARKING: All characters shall have a minimum height of 3/8 inch. Each carton shall have the following information displayed on two outside walls in addition to other required markings: 1. Contents: Trimedlure; 2. USDA, APHIS, PPQ 3. Up; 4. Do Not Crush; 5. Store in a dry place; 6. Quantity____ 7. Contract Number; 8. Date of Manufacture; 9. Manufacturer's Name; 10. Manufacturer's Lot No.; 11. Storage Instructions SPECIALMARKING: Each package/container of liquid trimedlure shall be clearly marked with the contract number & lot number. Each package shall be marked with the contract & batch numbers. The package labeling shall also include the lot number of trimedlure that was used in the production of this batch of dispensers. Reference Website: http//www.arnet.gov/far/fac.html (FAC 97-06) for Offeror Representations and Certifications-Commerical Items, complete, sign and return with the offer; and http//www.gsa.gov/forms/far.htm for required Standard Form 1449. Offeror shall submit the evaluation factors with the offer. The provisions FAR 52.212-1, Instruction to Offerors Commerical Items (Aug 1998); FAR 52.212.2 Evaluation -- Commerical Items (OCT 1997); FAR 52-212.3, Offeror Representation and Certifications-Commerical Items (OCT 1998); FAR 52.212- 4, Contract Terms & Conditions Commerical Items (APR 1998); FAR 52.212-5, Contract Terms and Conditions Required to Implement Statutes or Executive Orders-Commerical Items (OCT 1998) apply to this acquisition. Posted 12/10/98 (W-SN279355). (0344)

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