National Institutes of Health, National Heart, Lung, and Blood Institute, Contracts Operations Branch, 6701 Rockledge Drive, Room 6100, MSC 7902, Bethesda, MD 20892

A -- NOVEL HUMAN ORAL AND CRANIOFACIAL GENES SOL NHLBI-DR-99-18 DUE 030899 POC Patricia Smith, Contracting Officer, 301-435-0345 E-MAIL: Click here to contact the Contracting Officer via, ps44b@nih.gov. The National Heart, Lung, and Blood Institute (NHLBI) is soliciting proposals on behalf of the National Institute of Dental and Craniofacial Research (NIDCR) for services to characterize novel human genes expressed preferentially in embryonic rhombomeres and first branchial arch, oral cavity (including teeth, salivary glands and tongue), and maxilla. This research will include the following: 1) Produce a set of accurately staged tissue-specific and developmental stage-specific human craniofacial cDNA libraries, with documentation concerning the tissue (source and quality), embryonic stage, and quality of the library. 2) Libraries and RNA corresponding to the same stages or specific tissue used for these libraries shall be provided to the NIDCR Project Officers. In addition, at least four developmental stage-specific or tissue-specific standard cDNA libraries shall be provided to the I.M.A.G.E. Consortium for high-throughput sequencing, including at least one library from a very early stage. 3) Apply screening approaches to compare the genes expressed in the different human craniofacial embryonic stages or tissues, in order to generate information that researchers in the field can use to obtain and characterize cDNA clones that are developmental stage-specific and tissue-specific. 4) Identify and isolate stage-specific (particularly from early stages) or unusually highly expressed tissue-specific clones. 5) Information generated by this contract, including DNA sequences and summaries of database searches, shall be provided to the Project Officers in a form ready for deposit in an NIDCRI World Wide Web page in order to make the data freely available. The period of performance is three years. One award is anticipated to be made in September 1999. The RFP will be issued on or about February 5, 1999, with proposals due March 8, 1999. The RFP will be available electronically on the NHLBI Web Page at: http://www.nhlbi.nih.gov/nhlbi/rafs.htm#rfp. There are no paper copies. Interested organizations should review and download the streamlined RFP designated as RFP # NHLBI-DR-99-18 which will appear under the list of RFPs for the National Heart, Lung, and Blood Institute. Further instructions and forms for proposal submission are located at the menu selection entitled "Streamlined RFP References" which is located at the web page for the NIH Request For Proposals Directory, URL: http://www4.od.nih.gov/ocm/contracts/rfps/mainpage.htm. Posted 01/19/99 (W-SN289115). (0019)

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