DHHS/PHS/FDA/Office Of Facilities, Acquisition, And Central Services, Division of Contracts and Program Management, HFA-512(COB), 5600 Fishers Lane, Rockville, MD 20857

A -- MOLECULAR BIOLOGIST TO MONITOR TRANSGENE STABILITY AND TUMORIGENIC RESPONSE MECHANISMS IN TRANSGENIC MICE SOL E30988-00-99 DUE 030199 POC Trudy Dartouzos, Contract Specialist, ph: 301/827-7165, fax: 301/827-7103 See Note #1. The Food and Drug Administration [FDA], Center for Drug Evaluation and Research [CDER] intends to award a purchase order for a Molecular Biologist to be responsible for monitoring transgene stability and tumorigenic response mechanisms in transgenic mice. The contractor must have completed all requirements for a Bachelor of Science degree from an accredited university and graduates of foreign universities must submit certification of education. Two years of previous relevant moleular biological research experience is required. The contractor shall be expected to provide technical services for studies that measure toxicities of agents to animals or to normal or neoplastic cells. Services shall be a technical component of studies of a broader scope in which a research team or group is examining a significant problem or question from several perspectives. The project officer will guide and advise the contractor to execute portions of the research effort. The contractor must demonstrate a knowledge of cellular and animal biology, physiology, chemistry and molecular biology, in addition to skills in tissue culture, assay performance, analytical molecular biological procedures and ex vivo analysis of animal tissues. The contractor shall have knowledge of the requisite procedures for handling biohazardous, chemical, and radiological materials in the lab and the use of personal computers for data reporting and retrieval for information retrieval and for written commuication as needed. The contractor shall be responsible for detailed execution of assigned experiments using identified techniques and methods which may need further development or evaluation and be expected to consult with the project officer or his designee during any phase of work if necessary, but will be given the latitude to schedule experiments to meet indicated deadlines. The end result of the experiemental work shall be reliable and reproducible with respect to accuracy and technical soundness as well as clear, concise experiemental data in both written and oral form on a timely basis. Statement of Work: 1] prepare necessary solutions, buffers, cell culture media and any other materials required to perform designated studies, 2] collect blood and/or other animal tissues, 3] use in vitro cellular approaches which are in the process of being developed for the purpose of evaluating the toxicity and efficacy of new pharmacologic agents to primary and transformed cells [e.g., micro titer assays, etc.], 4] perform a variety of molecular biological techniques [e.g., DNA and mRNA isolations, gel electrophoresis, Southern or Northern blotting, membrane hybridizations with radiolabeled probes, PCR amplifications, DNA sequencing, etc., 5] gather, organize and present scientific data to project officer and team written or orally, 6] assist in the analysis of data, and 7] decontaminate and clean materials and work areas used in the course of the studies. The requirements for the Statement of Work shall include performing all facets of the studies or be limited to specialized technical support procedures. Performance Standard: Contractor shall comply with all technical research service requirements, including safety and animal regulations, to insure the integrity of research. Period of performance: one year from date of award and all work shall be on-site -- Laurel, Maryland. Written quotes along with a resume or curriculum vitae are required by 2:00 pm, 3/1/99. Posted 02/12/99 (W-SN297866). (0043)

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