U.S. Environmental Protection Agency, 26 W. Martin Luther King Dr., Cincinnati, OH 45268

66 -- IMAGE ANALYSIS SYSTEM (LUMI-IMAGER SYSTEM) SOL 99-84 DUE 031099 POC Mike Hennessey, 513-487-2032 E-MAIL: EPA Contracts Management Division, Cincinnati, OH, hennessey.mike@epamail.epa.gov. IMAGE ANALYSIS SYSTEM (LUMI-IMAGER SYSTEM) This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in FAR Subpart 12.6, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; proposals are being requested and a written solicitation will not be issued. Solicitation number 99-84 is issued as a request for quotation. The solicitation document and incorporated provisions and clauses are those in effect through Federal Acquisition Circular 97-09. This is a total small business set-aside. System Description: An Image Analysis System is needed for human biomarker research which must give optimal chemiluminescent or fluorescent image and able to detect the signals with high sensitivity and precision and show low background for assaying human tissues and body fluid in a wide range of applications, including applications for the formats of gel, blots and 96-well mirotiter plates. The image system shallcontain a work station for detection and quantification of chemiluminescence and fluorescence with a two year warranty and two copies of software for graphics and image analysis, and the vender shall provide full instruction documents and on-site training needed. The workstation includes a cooled, high-performance CCD camera in a dark room enclosure with a high-performance, focus-stabilized sensitive lens for the detection of very low level light signals, a UV transilluminator as an excitation source for fluorescence, an automatic filter wheel, a sample holder, a pentium personal computer with a color monitor, a laser printer, Windows operating system, and the imaging software which is easy to use. Minimum Performance Specifications: 1) Wide applications for analyzing samples in various formats, including gels (at least 20x20cm), blots (at least 24x30cm) and microtiter plates; shall be able to place 3 microtiter plates or more at one time in the sample holder. 2) High sensitivity and precision with low background for detection and quantification of chemiluminescence and fluorescence, assure the accuracy of data, and reproducible results: -- equipped with a CCD camera with large pixel size of 16x16 m and have chip size of 1280 x 1024 pixels and provide wide range dynamic range of 1:10,000; shall be a 50mm focus-stabilized slow scan camera; lens shall have aperture of 0.95; low distortion of <1 pixel; provide high resolution of 2 line pairs/mm and able to analyze the closely spaced bands separated by <1mm; with low vignetting (20% at edges before correction); shall have triple stage peltier cooling to -35 C for low thermal noise (0.06-0.18 electrons/sec). -- fluorescence shall be equipped with high energy UV transilluminator; excitation peak at 312 nm; shall equip with a filter Wheel with at least four positions for fluorescent detection which can detect the common fluorescent stains, including ethidium bromide, SYBR Green, SYPRO Red, fluorescein, AMCA, and rhodamine; able to detection at 600 20 nm, 520 20 nm, and 645 7.5 nm. -- software shall be able to perform automatic correction for pixel variation of the dark current, biase correction, cosmic ray correction and flat-field correction and piexel defect correction. -- can perform image analysis to 0.3% accuracy both in mass molecular weight or base pair determination. -- easy to perform with automation, do not require to change the lens or to focus the instrument -- software shall be easy to use; able to analayze signals over 4 orders of magnitude and with high sensitivity; able to quantify signals to determine mass and molecular weight; create standard calibration curves; can also analyze data on the remote workstation. Quotes shall also include the price for: two copies of software; a two year warranty; shipping; installation; on-site training at the EPA Human Studies Facility, Chapel Hill, NC. F.O.B. point shall be destination and delivery shall be made to EPA Shipping & Receiving, I-40 & Page Rd. Research Triangle Park,NC 27711. Delivery shall be made no later than 30 days after award of the purchase order. The provisions at 52.212-1, Instructions to Offerors-Commercial, applies to this to this acquisition. The provision at 52.212-2, Evaluation-Commercial Items is applicable and the specific evaluation criteria are as follows: (1)Technical capability of the item offered to meet Government requirement and (ii) price. Offerors shall include a completed copy of the provision at FAR 52.212-3, Offeror Representations and Certifications-Commercial Items, with its offer. Clause 52.212-4, Contract terms and Conditions-Commercial Items applies to this acquisition. The Clause at 52.212-5, Contract Terms and Conditions required to Implement Statutes or Executive Orders-Commercial Items, applies to this acquisition, however, for paragraph (b), the following clauses apply to this acquisition: 52.222-26 Equal Opportunity; 52.222-35, Affirmative Action for handicapped Workers; 52.222-37 Employment Reports on Special Disabled Veterans andVeterans of the Vietnam Era; and 52.225-3 Buy America Act -- Supplies. Offers shall also include sufficient descriptive literature to demonstrate that the items offered meet the minimum requirements specified above. Offers shall be submitted no later than March 10, 1998 at 4:00 p.m. E.S.T. to Mike Hennessey at: regular mail: U.S. EPA, Contracts Management Division, 26 West Martin Luther King Drive, Cincinnati, OH 45268, or by FEDEX: U.S. EPA, 4411 Montgomery Road, Suite 300, Norwood, OH 45212. Questions or comments may be directed to Mike Hennessey at (513-487-2032), by e-mail (hennessey.mike@epamail.epa.gov). Posted 02/23/99 (W-SN301240). (0054)

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