U.S. Environmental Protection Agency, 26 W. Martin Luther King Dr., Cincinnati, OH 45268

66 -- FLOW CYTOMETER SOL RFQ-OH-99-00102 POC Kathleen Roe, Phone: (513)487-2144, Fax: (513)487-2109 WEB: Office of Acquisition Management -- Cincinnati Contracts, This procurement will not appear on the web site.. E-MAIL: U.S. EPA -- Cincinnati Contracts Mangement Division, roe.kathleen@epamail.epa.gov. The U. S. EPA National Exposure Research Laboratory has a requirement for a Flow Cytometer. The contractor shall provide a system having the following capabilities: (1) Minimum of 5 detectors: forward scatter, side scatter, and 3 epifluorescence detectors; (2) Minimum capability of 3 lasers lines simultaneously. For example, using a laser that provides 180 mW power at 488 nm and 50 mW at 351-364 nm with beam splitter to provide UV/488 illumination and a laser that provides 35 mW at 633 nm. Requires three laser slots, one for UV, one for 488, and one for the HeNE or second laser; (3) Computer for analysis of minimum 48 megabytes RAM, 2 megabytes VRAM, and 2 gigabyte hard drive, with 10 Base-T Ethernet connector, 20" color monitor; (4) Calibration beads; (5) Installation, training, and 1 year warranty; (6) Sorting by electromagnet charge sorting, NOT mechanical sorting; (7) Ability to sort on at least 5 detectors simultaneously; (8) Ability to adjust and align all lasers, using standards; (9) Ability to sort using non-rectangular sort windows; (10) Ability to adjust side stream and droplet rate; (11) Ability to sort at high speed; (12) Intensive training course for a minimum of two individuals; (13) Flexibility in triggering and gatin; (14) Simultaneous multiparameter analysis; (15) Software package that allows multivariate analysis and counting concurrent to sorting, and multivariate analysis and counting after sorting, allowing generation of publication quality output including: histograms, and dot plots; (16) System shall be listed and labeled by a third party listing agency approved by the NC Building Code Council. For further specifications see "Practical Flow Cytometry, Third Edition" by Howard M. Shapiro, Wiley-Liss Inc., NY, 1995, ISBN 0-471-30376-3. This procurement will be processed in accordance with FAR Part 12, Acquisition of Commercial Items. Requests for RFQs must be mailed, faxed, or e-mailed to the above POC not later than 3/22/99. Please include a complete mailing address and fax number. All quotations received by the date stated in the RFQ will be considered by the Government. Posted 03/05/99 (W-SN305539). (0064)

Loren Data Corp. http://www.ld.com (SYN# 0362 19990309\66-0010.SOL)