National Cancer Institute, Research Contracts Branch, PSAS, 6120 Executive Blvd, EPS/Room 638, Bethesda, MD 20892-7227

66 -- SIS PACKAGE SOL RFQ90054 DUE 032599 POC Patricia Haun, Purchasing Agent, 301-402-4509 The National Cancer Institute (NCI), on behalf of the National Institute of Neurological Disorder and Stroke (NINDS), Laboratory of Neurobiology (LN), plans to procure image acquisition and analysis software, as well as related hardware, cables and interfaces, that will interface to its slow-scan charge coupled device (CCD) camera from Leo Electron Microscopy, Inc., One Zeiss Drive, Thornwood, NY 10594. The LN's current instrument for fundamental cell biology research is a LEO 912 Omega energy-filtering electron microscope, whose capabilities for low-dose, quantitative imaging, energy filtering, and cryoelectron microscopy would be greatly expanded by a suitable software capable of taking advantage of this microscope and its associated high-sensitivity CCD camera system. The software, as well as related essential hardware, cables and interfaces are to be installed on and interfaced to the existing Proscan Vario-Speed 2MHz slow-scan CCD camera system. The combination of these instruments will expand the capability and improve the convenience of recording data from rapidly frozen neuronal specimens, leading to an increase in research productivity. The software package that is required, in addition to being compatible with, installed on, and interfaced to the existing Proscan Vario-Speed 2MHz slow-scan CCD camera system, shall include the following: 1) an ESIVISION software package (SIS GmbH) consisting of driver Pro, FFT, Spectrum analysis tools; 2) EFTEM acquisition and analysis (including standard EELS, parallel EELS, image EELS, and ESI elemental mapping); 3) SIS software shall be version 3.0 or higher and fully 32-bit, Windows 95/98/NT compliant, and Y2K compliant; 4) SIS Pentium II/MMX PC (minimum configuration 128MB RAM, 10GB hard disk); 5) Grabbit PCI framegrabber card; 6) two high-resolution 17" monitors with interfaces; 7) TTL and RS232 interfaces and the TEM remote software library for microscope communication; 8) on-site installation and set up to meet the required performance. In addition, the SIS ESIVISION software package shall provide optimized and direct control of the microscope operating parameters, a suite of software tools for acquisition and processing of energy-filtered EM images, and Windows/PC-based custom software routines for microscope control. The SIS ESIVISION software is the only software system that is compatible with the hardware and firmware environment of our existing Proscan Variospeed 2MHz slow scan CCD camera. Only this software is known to yield a typical noise level (under low illumination conditions) of only one electron per readout, thereby delivering the required level of performance with the complete system. Likewise, the SIS ESIVISION software package provides other unique features, including optimized direct control of operating parameters, tools for acquisition and processing of energy-filtered EM images, as well as control and processing software routines. Leo Electron Microscopy, Inc., is the only source known to NINDS that can meet the above requirements and provide an image acquisition and analysis software that is fully compatible with the mechanical, hardware, and software environment of the existing CCD camera system and LEO 912 microscope. However, if any interested party believes it can meet the above specifications, it may submit a statement of capabilities. The statement of capabilities and any other information furnished must be in writing and must contain material in sufficient detail to allow NCI/NINDS to determine if the party can perform the requirement. Capability statements must be received in the contracting office by 1:00 p.m. EST, on March 25, 1999. If you have any questions, please contact Patricia Haun on (301) 402-4509. A determination by the Government not to compete this proposed contract based upon responses to this notice is solely within the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. Posted 03/08/99 (W-SN305869). (0067)

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