NASA/Ames Research Center, JA:M/S 241-1, Moffett Field, CA 94035-1000

66 -- HIGH PRESSURE LIQUID CHROMATOGRAPHY SYSTEMS SOL RFQ2-36689-SLC DUE 032999 POC Sharon L. Connolly, Contracting Officer, Phone (650) 604-6927, Fax (650) 604-4646, Email sconnolly@mail.arc.nasa.gov -- Barbara A. Drake, Contracting Officer, Phone (650) 604-3002, Fax (650) 604-4646, Email bdrake@mail.arc.nasa.gov WEB: Click here for the latest information about this notice, http://nais.nasa.gov/EPS/ARC/date.html#RFQ2-36689-SLC. E-MAIL: Sharon L. Connolly, sconnolly@mail.arc.nasa.gov. This notice is a combined synopsis/solicitation for commercial items prepared in accordance with the format of Federal Acquisition Regulation (FAR) Subpart 12.6, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; quotes are being requested and a written solicitation will not be issued. This procurement is being conducted under the Simplified Acquisition Procedures (SAP). NASA Ames Research Center intends to purchase two (2) complete High Pressure Liquid Chromatography Systems for protein purification and biochemical fractionation of complex biological samples. Because these systems will be used for separation of a wide variety of materials, they must be completely configurable for separation on the basis of time, volume, or column-volume. These apparatus require a two-pump gradient system using dynamic mixing to permit creation of very shallow gradients, with motorized valves that provide feedback to the control system to ensure proper orientation of all valves and documentation of valve positions at all times. The systems shall include UV, conductivity, and pH sensors, an integrated fraction collector, and a controller with preprogrammed control software and documentation that permits full automation of sample runs. The system must be fully inert to standard biological buffers, and be certified for operation from 4-40C in 10-95% relative humidity. A system flow rate of 0.05-20ml/min is required, with operating pressures from 0-5 MPa (50bar, 725psi) and user-defined programmable pressure cutoff limits. The control software must have the capability to record both predicted and actual conductivity as well as back pressure, pH and UV absorbance. The conductivity sensor's range is to be 1 microS/cm -- 999.9 mS/cm with noise of less that 0.5% of full scale calibrated range. A pH sensor range of 0-14pH units is required with accuracy within 0.1pH units and drift of less than 0.1pH units/10hr. In addition, the pH sensor is to include a dummy flow cell for use with organic solvents that might harm the pH electrode if in direct contact. The UV sensor must permit selection of wavelengths at 245 and 280nm, be linear within 3% up to 2 absorbance units (AU) at 254nm, and linear within 5% up to 1AU at 280nm, with noise of less than 40x10^-6 AU and drift of less than 100x10^-4 AU at 254nm. The offered package must include two (2) complete systems with pH electrodes and computerized control devices, one (1) color documentation system, one (1) 50ml sample loading loop with required cables, and four (4) chromatography columns: two high-resolution columns for anion or cation exchange, a fast desalting column, and a high resolution gel filtration column for separation of microgram-milligram sample quantities. Installation, training and standard warranty are to be provided at no additional cost. The provisions and clauses in the RFQ are those in effect through FAC 97-10. The Standard Industrial Classification (SIC) Code and size standard for this procurementare 3826 and 500 employees, respectively. The quoter shall state in their quotation their size status for this procurement. All qualified responsible business sources may submit a quotation which shall be considered by the agency. Deliver to NASA Ames Research Center, Receiving Section, Bldg. 255, Moffett Field, CA 94035 is required within 45 days after Purchase Order award. Delivery shall be FOB destination. The DPAS rating for this procurement is DO-C9. Quotations for the item(s) described above may be mailed or faxed to the identified point of contact by the date/time specified and include, solicitation number, FOB destination to this Center, proposed delivery schedule, discount/payment terms, warranty duration (if applicable), taxpayer identification number (TIN), identification of any special commercial terms, and be signed by an authorized company representative. Quoters are encouraged to use the Standard Form 1449, Solicitation/Contract/Order for Commercial Items form found at URL: http://procure.arc.nasa.gov/Acq/Forms/Index.html to submit a quotation. Quoters shall provide the information required by FAR 52.212-1. Addendum to FAR 52.212-1 is as follows: Compliance with Veterans' Employment Reporting Requirements (February 1999) By submission of its offer, the offeror represents that, if it is subject to the reporting requirements of 37 U.S.C. 4212(d) (i.e., the VETS-100 report required by Federal Acquisition Regulation clause 52.222-37, Employment Reports on Disabled Veterans and Veterans of the Vietnam Era), it has submitted the most recent report required by 37 U.S.C. 4212(d). If the end product(s) quoted is other than domestic end product(s) defined in the clause entitled "Buy American Act -- Supplies," the quoter shall so state and shall list the country of origin. The Representations and Certifications required by FAR 52.212-3 may be obtained by phone or fax to the primary point of contact, Sharon L. Connolly at the address/email stated below. FAR 52.212-4 is applicable. FAR 52.212-5 is applicable and the following identified clauses are incorporated by reference: 52.222-3, 52.233-3, 52.222-21, 52.222-26, 52.222-35, 52.222-36, 52.222-37, and 52.225-3. Addenda to FAR 52.212-5 are as follows: ARC 52.232-93 Submission of Invoices-Fixed Price (Feb 1998) and clause "Year 2000 Compliance (May 1998)" in accordance with PIC 98-8. The quoter shall provide standard product literature indicating that the product or products offered are Year 2000 Compliant. Questions regarding this acquisition must be submitted in writing no later than March 22, 1999. Quotations are due by 3:30pm PST on March 29, 1999, to the attention of Sharon L. Connolly and should be mailed to NASA-Ames Research Center, M/S:241-1, Moffett Field, CA 94035. It is the quoter's responsibility to confirm if the Government has received the faxed quotation. Selection and award will be in accordance with FAR 52.212-2 with the following evaluation factors: (1) technical acceptability, (2) past performance, and (3) price. Technical acceptability will be determined using information submitted by the quoter in sufficient detail to show that the product quoted meets the Government's requirements. Quoters must provide copies of the provision at 52.212-3, Offeror Representations and Certifications -- Commercial Items with their quote. An ombudsman has been appointed -- See Internet Note "B". It is the quoter's responsibility to monitor this site for the release of amendments (if any). Potential quoters will be responsible for downloading their own copy of this combination synopsis/solicitation and amendments (if any). Any referenced notes can be viewed at the following URL: http://genesis.gsfc.nasa.gov/nnotes.html. Posted 03/17/99 (D-SN309799). (0076)

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