U.S. Environmental Protection Agency, 26 W. Martin Luther King Dr., Cincinnati, OH 45268

66 -- GEL AND BLOT ANALYSIS DUE 041599 POC Sandra Licis, USEPA, 26 W. Martin Luther King Dr. 45268, requests in writing Technical Specifications for Gel and Blot Analyses 1. One system must be capable of scanning, via software control, storage phosphor screens (autoradiography), red and blue excited fluorescent of DNA and protein gels, and chemifluorescent DNA/RNA and protein blots. 2.The selection of the appropriate optical components must be automatic when one of the three scanning modes is selected. System must use storage phospor screen technology, not flow through proportional counting. 3. Storage phosphor screens must be available unmounted in standard film cassettes and mounted on aluminum backing. 4. System must be compatible with existing Phosphorlmager storage phosphor screens and cassettes used for Molecular Dynamics 400E Phosphorimager. 5. System must be able to utilize 35x43 cm and 20x25 cm format mounted and unmounted storage screens, as well as gels and blots. 6. Scanning resolution must range from 200 to 50microns in software selectable units, with a beam diameter as small as 50 microns. 7. Uniformity must be plus or minus 5% relative standard deviation, and pixel accuracy plus or minus 0.15%. 8.Linearity must be less than 7.5% relative standard deviation for the entire dynamic range. 9. Linear dynamic range must be 5 orders of magnitude. 10. Light measurement for storage phosphor, fluorescence, and chemifluorescence must be collected and converted to electrical signal by a photomultiplier detector. 11. Light source is desireable to be a laser diode at 635 nm and an LED at 450 nm, with average lifetime of at least 50 thousand hours. 12. Detection thresholds: lower limit for one hour exposure must be less than 2 dpm/mm squared for Carbon-14(for 200 and 100 micron pixel size), with lower limit for detection of Phosphorous-32 usually at least five times lower. 13. Two excitation sources in one instrument: a. 635 nm excitation source for scanning phosphor screens and red-excited florescent samples. b. 450 nm excitation source for blue excited fluorescence and chemifluorescent samples. 14.Would like one software program to control the instrument, scan the sample,acquire the image, and analyze the data from all three scanning modes. 15. Software to perform operations identified in requirement 14, must be ab;e tp run on Macintosh PowerPC computers with G3 and 604e 200 MHz CPUs. 16/Updates to software must be provided at no additional charge during the warranty period. Warranty must be covered by at least one year. 17 System must include an image-eraser box for all size storage screens used by the system. 18 System and image-erasure box must operate on 50/60 Hz and 115/230V (switchable) with power draw of no more than 500 watts for the system and 150 watts for the image erasure. 19. System and image-erasure dimensions in inches should not exceed: height width depth system 20 35 31 image-erasure 30 20 31 All interest must be requested in writing NLT 4/15/99. Posted 04/01/99 (W-SN315451). (0091)

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