U.S. Department of Commerce/National Oceanic and Atmospheric Administration/OFA/AGFS/AMD -- OFA51, 1305 East West Highway -- Station 7604, Silver Spring, Maryland 20910

B -- CHEMICAL/BIOLOGICAL STUDIES AND ANALYSES SOL 9-4159 DUE 041999 POC Sandra Souders (301) 713-0854 B -- The U.S. Dept. of Commerce/NOS intends to negotiate on a sole source basis with Columbia Analytical Services (CAS), located in Vista, California, to conduct testing for NOAA with the unique Biomarker, P450 Reporter Gene System (RGS), composed of a human liver cancer cell line genetically engineered to produce the luminescent enzyme, luciferase, when toxic and carcinogenic organic compounds are present. The contractor (CAS) will test sediment samples collected by NOAA and its collaborators as part of sediment toxicity studies in Puget Sound (100 samples) and Chesapeake Bay (60 samples). Samples will be collected from May to September, 1999. The P450 RGS Testing of Sediments includes the following: 1) Analyzing sediment samples using the P450 Reporter Gene System (RGS) bioassay; 2) Providing a portion of sediment extracts to other NOAA contractors for additional analyses; 3) Performing chemical analyses on a few selected samples that show unusually high RGS response; 4) Reporting the results. This is a notice of intent with the award going to Columbia Analytical Services if no other favorable responses are received within 15 days of this notice. No solicitation package exists; therefore, a request for such package will not be considered. Award will be made under Simplified Acquisition Procedures. (Reference No. 9-4159) Posted 04/02/99 (W-SN315757). (0092)

Loren Data Corp. http://www.ld.com (SYN# 0017 19990406\B-0005.SOL)