Acquisitions Management Operations Branch, NIAID, 6003 Executive Blvd, Solar Building, Room 1C38, Contracts Section, Bethesda, MD 20892-7605

R -- OPERATION AND MAINTENANCE OF A MURINE PATHOGEN-FREE MOUSE BREEDING FACILITY SOL NIAID-DIR-99-53 DUE 063099 POC Karin Eddy (301) 496-3878 The Division of Intramural Research, National Institute of Allergy and Infectious Diseases has a requirement to establish, operate and maintain a murine production colony under strict double barrier conditions to contain approximately 2400 cages of inbred, congenic, transgenic and DNA-mediated gene targeted mutant mice and their offspring. Any offeror must have documented successful experience in the operation of a high quality breeding facility for the types of mice mentioned above. Any offeror shall provide a minimum of two completely autonomous foundation colonies for actively maintained strains to provide backup in the event of a pathogenic contamination. In addition the successful offeror shall maintain a separate barrier unit of 1200 cages of mice for production purposes. The building construction of the barrier units must be in compliance with the publication "(The Guide for the care an Use of Laboratory Animals" (The Guide) and be equipped with an air handling system including HEPA air-filtration. These barrier facilities must be dedicated exclusively to the contract. The offeror shall provide all caging, feed, water, and miscellaneous supplies necessary to maintain the colony. The offeror must provide personnel experienced in the interpretation and performance of complex breeding protocols and having the ability to sample a variety of tissues which will be sent to NAID for genotyping. The offeror must have the proven capability to re-derive mice by embro transfer or Cesarean section, establish defined micro-flora foundation and production colonies and cryopreserve and reconstitute mouse embroyos. The offeror shall maintain a physically separate and independently staffed quarantine area to house mice prior to re-derivation. Contractor must be AAALAC accredited and provide veterinary care and a complete health monitoring program overseen by an ACLAM Certified Veterinarian specializing in small rodents. The offeror shall deliver animals under climate controlled conditions in keeping with standards specified in The Guide. The offeror must be experienced with and provide a computerized relational data base capable of keeping breeding, performance and pedigree records with access to e-mail distribution. The offeror must be able to provide sales, distribution, technology licensing and accounting services necessary to market mice for the NIAID transgenic "Exchange" program. It is proposed that any contract issued will be a 7 year cost reimbursement completion type contract using incremental funding. SEE NUMBERED Note 1. The RFP will be issued on or about May 20, 1999. All requests for the RFP must be in writing directed to Karin E. Eddy, Contracting Officer, Acquisitions Management Operations Branch, National Institute of Allergy & Infectious Diseases, Solar Bldg. Room 1C38, MSC 7605, Bethesda, Md 20892-7605. Posted 04/07/99 (W-SN317405). (0097)

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