U.S. Dept. of Commerce, NOAA, WASC, Acquisition Management Division WC3, 7600 Sand Point Way NE, Bin C15700, Seattle, WA 98115-0070

R -- PREPARE REPORT SUMMARIZING THE 1999 ANALYSIS OF DATA OBTAINED FROM SEQUENCING SITE STELLER SEA LION GENOME MATERAIL, AND INTERPRETATION OF THESE DATA IN PERSPECTIVE WITH PREVIOUS GENETIC RESEARCH ON STELLER SEA LIONS. SOL WASC9-1033 DUE 051499 POC Randall Brown (206) 526-6226/FAX (206) 526-6025 Sources sought to conduct genome analysis to detect nuclear gene variability. Assess the utility of nuclear primer sets by analysis of approximately 50 samples for six microsatelite loci. Conduct genome analysis by amplifying NTDNA using polymearse chain reaction technology and automatic sequencing to perform approximately 16 sample runs from approximately 50 samples supplied by the National Marine Fisheries Service (NMFS). Currently, the only known source to conduct analysis of sea lion tissue for assessment of available genetic primers to distinguish nuclear DNA, and for mtDNA sequence analysis, is the Texas A&M University (A&M). A&M has developed a technique to perform PCR/autosequence analysis on Steller sea lion tissues and identify those tissues to the island of origin. Period of performance under this contract will be for one year, with one option year subject to availability of funds. Any sources which have capable of providing such analyses are invited to request solicitation in writing by fax. This is a simplified acquisition with an estimated value of less than $100,000. Posted 04/20/99 (W-SN322421). (0110)

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