National Institutes of Health, National Heart, Lung, and Blood Institute, Contracts Operations Branch, 6701 Rockledge Drive, Room 6100, MSC 7902, Bethesda, MD 20892

66 -- INSTRUMENTS AND LABORATORY EQUIPMENT SOL NHLBI-PS-99-450 DUE 051599 POC Cecilia D. Morales, Purchasing Agent, (301)435-0369, Debra C. Hawkins, Chief, Procurement Section, (301)435-0366 The National Heart, Lung and Blood Institute (NHLBI), intends to purchase the Brand Name or equivalent Lightcycler Instrument with desktop computer, manufactured by Roche Molecular Biochemicals. The specifications for the Lightcycler are as follows:1.5 mm (O.D.) glass capillary Sample Format; Sample Capacity 10 -- 20 l for Sample Volume; the Sample Holder/Carousel should consist of Autoclavable, 32 sample capacity, constructed of precision-milled PEEK plastic for high thermodynamic stability and low heat conductance, and should be removable for easy loading, cleaning and sterilization; the Footprint size must be 30 X 45 X 30 (cm) and weight approximately 15 kg; the Detection Channels must be 3; the Detection Filters are 530 nm, 640nm, and 710nm; the Cycle Time must be capable of performing 30 cycles in less than 25 minutes and maximum temperature ramping time 20 C/second; the software must be Windows NT as the Operating System, must allow multi-tasking between windows and/or programs during sample runs, capable of performing a melting curve analysis for mutation detection and/or product verification; the input power must be selectable of 110 V, 115V, 220V, and 240V; the Heating Chamber must include Heating Coil, High speed-fan assemblies and cooling body. Desktop Computer and Peripherals: Hewlett Packard, 400 Mhz Pentium II PC, 17" Monitor, 64 MB Ram, 2 MB VRAM, 4.0 GB Hard Drive, 3.5", 1.4 MB floppy disk drive, CD-ROM drive, 100 MB internal Zip Drive, Soundcard, Hewlett Packard Inkjet 720C color printer and MS-DOS and Windows NT Systems. Motor: Low-maintenance, Belt-driven and Precision positioning of samples over optics. A separate price list must be submitted for the following Kits and Reagents: DNA Master SYBR Green I kit, DNA Master Hybridization Probes kit, Control DNA kit, RNA Amplification SYBR Green I kit, RNA Amplification Hybridization Probes kit, Control RNA kit, Red-640 NHS Ester Vial (for >250nmol of oligonucleotide), Capilaries set, Sample Carousel unit and Centrifuge Adapters and Cooling Block unit. The manufacturer instrument warranty should include complete coverage for all parts and labor, technical assistance hotline for hardware, software and application chemistry within 48 hour response time from the customer telephone call for on-site repair and or replacement. The Standard Industrial Classification (SIC) code is 3826, size standard 500. There are no set aside restrictions for this requirement. Quotations are due fifteen (15) calendar days from the publication date of this synopsis or May 15, 1999, whichever is later at 1:00 P.M. Eastern time. Quotations must include an itemized price, delivery time, prompt payment discount term, SIC code, Business size, DUNS number, literature and detailed specifications describing the product offered. Quotations will be evaluated on conformance to the government's specifications, quality, price, and delivery time. All responsible sources may submit a quotation which if timely received shall be considered by the agency. Quotations must be submitted in writing to the National Heart, Lung, and Blood institute, Contracts Operations Branch Procurement Section, Rockledge Building 2, Room 6143, 6701 Rockledge Drive, Bethesda, MD 20817-7902, attention: Ms. Cecilia D. Morales, Fax (301)480-3345. Fax quotations will only be accepted if dated and signed by an authorized company representative Posted 04/28/99 (W-SN325326). (0118)

Loren Data Corp. http://www.ld.com (SYN# 0621 19990507\66-0003.SOL)