National Institute of Environmental Health Sciences, Acquisitions Management Branch, 79 T.W. Alexander Drive, Building 4401 Research Commons, P.O. Box 12874, Research Triangle Park, NC 27709

66 -- DELAYED EXTRACTION MATRIX ASSISTED LASER DESORPTION TIME-OF-FLIGHT MASS SPECTROMETER SOL 273-99-P-0013 DUE 071899 POC Contact George Earle, Contracting Officer, (919)541-0377 The NIEHS is interested in obtaining a MALDI/TOF Mass Spectrometer as indicated in 8. above. This is a combined synopsis/solicitation for commercial items, and is prepared in accordance with the format in Subpart 12.6, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; proposals are being requested and a written solicitation will not be issued. The Request for Proposal number is 273-99-P-0013 and incorporates provisions and clauses through FAC 97-11. The solicitation is not set aside and is under SIC 3826, size 500. Delivery must be made within 90 days after award of the contract. Specifications are as follows: 1) delayed extraction capabilities; 2) linear and reflector operation modes possible; 3) positive and negative ion capability; 4) detector (microchannel plate) optimized for high mass detection; 5) low mass gate to filter out matrix ions; 6) resolution (full width half mass) greater than 10,000 over the mass range 1000-5000; 7) mass accuracy: less than 10 ppm error at mass 2300-2700 using an internal standard and less than 50 ppm error using an external calibration; 8) reproducibility: ten sequential acquisitions of at least 32 summed shots, each should not have a standard deviation greater than 5%; 9) sensitivity: ten femtomoles of a peptide in the 1000-2000 mass range should produce a signal-to-noise of greater than 10:1 with a resolution of 10,000 or greater; 10) a one year warranty; 11) software: If data files are not acquired under a Windows or NT operating system, the data files must be readable and manipulatable on a computer running a Windows or NT data system, i.e., data work-up software must be able to run under Windows or NT ; 12) Site license for the software must be included. The following provisions apply to this acquisition: 52.212-1 Instructions to Offerors -- Commercial Items and 52.212-2 Evaluation -- Commercial Items. There are no additional addenda. The following evaluation criteria will be used to evaluate proposals. 1. Sensitivity and resolution: a) three solutions of a tryptic digest of a protein isolated from an SDS PAGE gel will be provided. Each solution will be at a different concentration. One microliter of each solution should be spotted and 64 laser shots summed. The dried droplet matrix deposition method must be employed and alpha-cyano-4-hydroxy cinnamic acid used as the matrix. The volume of sample spotted and the number of laser shots summed must be indicated on the results. (34 points); b) A suite of peptides covering the mass range of 1000-10,000 Da will be provided. The peptides will not be present at the same level. Expanded plots of molecular ion regions should be provided to illustrate mass resolution. Mass assignment to 4 decimal places should be provided to illustrate mass accuracy. The volume of sample spotted and the number of laser shots summed must be indicated on the results. (33 points) 2) Two sets of peptide(s) immobilized on affinity material will be provided; one for positive ion and one for negative ion analysis. A 1 microliter aliquot of the affinity material mixture (after vortexing) should be spotted to the previously deposited (dried droplet) matrix (alpha-cyano-4-hydroxy cinnamic acid for positive ion and alpha-cyano-4-hydroxy cinnamic acid with 1 microliter of 1 mM ammonium acetate added for negative ion). Data should be provided in such a way that the resolution and mass accuracy of the results can be determined. All operating conditions should be indicated (33 points). All firms wishing to submit an offer will be furnished samples by overnight courier for analysis on the type of instrument proposed. Samples will be released to offerors at one time; therefore requests for samples must be requested by COB 11 June 1999 and released 16 June 1999. Sample analysis results shall be submitted with the proposal for evaluation. Furnish the name, address, telephone number and a contact name of at least three Gov't/Commercial customers which have purchased similar instruments in the last two years. This information will be used to evaluate past performance. Price will not be evaluated; however, if two or more instruments are within 10 points they will be considered technically equal and the lowest priced offer will receive the award. Offers shall include a completed copy of the certifications at 52.2123, Offeror Representations and Certifications -- Commercial Items with offers. Clause 52.212-4, Contract Terms and Conditions -- Commercial Items applies. The following sections of paragraph (b) of clause 52.212-5, Contract Terms and Conditions Required to Implement Statutes or Executive Orders -- Commercial Items apply: 1, 5, 9-15, and 23. The successful offeror shall provide installation and training to selected NIEHS employees. There are no CBD numbered notes that apply. Offers are due by COB 20 July 1999. For information contact George Earle (191)541-0377. ****** Posted 05/10/99 (W-SN329530). (0130)

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