National Institute of Diabetes, Digestive & Kidney Diseases (NIDDK), Acquisition Management Branch, Natcher Building, 45 Center Dr., MSC 6600, Room 6AN32, Bethesda, Maryland 20892-6600

66 -- GENECHIP HU6800 ARRAY AND GENECHIP HU35K SET PROBE ARRAYS SOL NIH-NIDDK-99-454 DUE 061499 POC Desiree Kelly, Purchasing Agent 301-594-7731 The National Institutes of Health (NIH) National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) has a requirement to purchase Genechip Hu6800 Array and GeneChip Hu35K Set probe arrays. The Institute intends to obtain this scientific equipment by other than full and open competition from Affymetrix Inc., 3380 Central Expressway, Santa Clara, CA 95051. The Standard Industrial Classification Code is 3821, and the business size standard is 500. To the best of the Government's knowledge they are the sole manufacturer and distributor of this equipment. The required specifications must be met for the Genechips 1) Expression levels of approximately 6800 full-length human genes are quantitatively detected on the GeneChip Hu6800 set. 2) Expression levels of approximately 35,000 human EST sequences are quantitatively detected on the GeneChip Hu35K set. 3) Each gene or EST is analyzed with approximately 20 pairs of specific, unique 25mer oligonucleotide probes. 4) Pairs of perfect and single-base mismatch probes are synthesized on adjacent areas on the arrays. Data analysis requires this probe pairing strategy so that nonspecific hybridization and background signal can be identified and subtracted. 5) Over 65,000 different oligonucleotide probe sequences are synthesized in-situ on a 12.8mm X 12.8 mm probe array. Each probe sequence is located in a specific area on the probe array called a probe cell, measuring approximately 50 microns by 50 microns. 6) Each probe cell contains approximately 107copies of a given probe. Sensitivity of detection is from a few copies to several hundred copies per cell. 7) Each array contains probes complementary to the bacterial genes bio b, bio c, bio d and cre, which are used as normalizat6ion controls. 8) Each array is packaged in a self -contained disposable cartridge which acts as a reaction chamber. Interested vendors may identify in writing their interest with a capability statement to the Contacting Office prior to the closing date of this notice. This notice of intent is not a request for competitive proposals, however all responses received before the closing date of this announcement will be considered by the Government. A determination by the Government not to compete this proposed acquisition is based upon responses to this notice and is solely within the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. It is anticipated that Request for Quotation (RFQ) NIH-NIDDK-99-454 will be available fifteen (15) days after the publication date of this synopsis. All responsible small business and 8(a) sources may submit a quotation which, if timely received, shall be considered by the agency. Receipt of quotations will be due ten days after the release of the RFQ. The anticipated award date is June 16, 1999. Request for copies must be in writing and may be FAX to 301-480-4226. Quotations must be submitted in writing to the National Institute of Diabetes and Digestive and Kidney Diseases Room: 6AN32, 45 Center Drive MSC 6600 Bethesda, MD 20892 Attention: Desiree Kelly FAX quotations must contain offerors signed by an authorized company representative. Posted 05/21/99 (W-SN334432). (0141)

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