USDA, ARS, Southern Plains Area, 7607 Eastmark Drive, Suit 230, College Station, Texas, 77840

66 -- FPLC CHROMATOGRAPHY SOL 3-6202-99 DUE 070699 POC Gloria Lopez, Contracting Officer, 409-260-9336, FAX 409-260-9589 E-MAIL: click here to contact the contracting officer via, g0l7342@acs.tamu.edu. This is a combined synopsis/solicitation for commercial items prepared in accordance with the format in FAR subpart 12.6, as supplemented with additional information included in this notice. This announcement constitutes the only solicitation; proposals are being requested and a written solicitation will not be issued. The solicitation number is 3-6202-99 and is issued as a request for quotation (RFQ). This solicitation document incorporates those provisions and clauses in effect through Federal Acquisition Circular 97 -- 09. The USDA, Agricultural Research Service request quotations for one (1) FPLC chromatography system used for the purification of biologically active molecules such as proteins and DNA. Specifications: (1) All wetted surfaces are composed of inert material such as PEEK, borosilicate glass or fluoroplastics. The system is compatible with all solvents commonly used for the purification of biologically active molecules. (2) The system pumps are syringe-type, and do not contain ball check valves. The flow range for the pump must be from 0.05 to 20 mL/min at pressures ranging from 0-725 psi. (3) The system controller is able to control a chart recorder, fraction collector, gradient mixer, air sensors, three pumps, and up to eight motorized valves. The system controller can also allow editing of methods and/or the simultaneous integrating and evaluating of results from earlier runs, while a current separation is being done. (4) System controller can accept input signals from monitors for UV wavelengths, pH, conductivity, temperature, and pressure. The system is capable of plotting and integrating these signals, as well as using them to trigger subroutines. (5) The system controller should permit automatic running of up to 99 different programs in a user-designated sequence. The controller must have post-run chromatographic analysis and reporting functions. (6) The system can have complete automation capabilities such as automatic column cleaning, multicolumn manifolding, methods and development scouting, reverse flow techniques, multiple and single automated sample injection, and injections from 10 microliters up to 150 milliliters of a sample without having to go through a pump. (7) The system controller has the following minimum specifications: 350 MHz Intel Pentium II computer, 100MHz Bus speed, 512 KB cache, 6.4 GB Ultra ATA hard drive, 3.5" 1.44" floppy drive, 24x CD-ROM drive, Iomega internal ZIP1000 drive (IDE version), ATI Rage turbo AGP 2X enhanced graphics, 4 MB video SGRAM -- ESS ES1869 embedded sound card, 104 key enhanced keyboard, PS/2 style mouse, V75 (17") monitor, Microsoft Windows NT 4.0 workstation. (8) The system UV/VIS monitor is capable of operating at 254, 280, 313, 405, 436, 546 nm, with an absorbance range from 0.001 to 2 AU. (9) The conductivity monitor has a sensitivity range from 1 microS/cm to 999.9 mS/cm, and can measure the temperature of the mobile phase. (10) The pH monitor has a range from 0 to 14 pH units. (11) The pumps and the modules for monitoring UV/VIS absorbance, conductivity, and pH have an operating temperature from 4 to 40 degrees C. (12) The contractor shall perform all delivery and set-up so that the FPLC chromatography system is complete and ready to use. The FPLC chromatography system shall be delivered to the USDA, Agricultural Research Service, Food Animal Protection Research Laboratory, 2881 F&B Road, College Station, TX 77845. The Government "desires" delivery within 30 days or less after receipt of the purchase order by the successful offeror; however, each offeror shall include their proposed delivery schedule as part of their quotation. Acceptance and FOB point are destination. In order to be considered for award offerors shall provide a quotation on company letterhead detailing the unit pricing; descriptive literature, brochures and references shall also be provided for the product quoted. The Government is conducting this procurement using a trade-off approach, where technical capability of the product to perform the desired function, past performance and price will be evaluated in making an award. The Government expressly reserves the right to award to other than lowest priced offer. The following FAR provisions apply to this solicitation: 52.212-1 "Instructions to Offerors -- Commercial", Offerors shall include a completed copy of the provision at 52.212-3 "Offeror Representations and Certifications -- Commercial Items", with this offer; a copy of this provision may be obtained from the contracting officer or at http://www.arnet.gov/far. 52.212-4 "Contract Terms and Conditions -- Commercial Items", 52.212-5 "Contract Terms and Conditions Required to Implement Statutes or Executive Orders -- Commercial Items". Furnish quotations to Gloria Lopez, Contracting Officer, USDA, ARS, FAPRL, 2881 F&B Road, College Station, Texas, 77845, no later than close of business July 6, 1999. Quotations my be provided by facsimile to (409) 260-9589 if desired. Additional information may be obtained by contacting the contracting officer at (409) 260-9336 (voice) or email below. Posted 06/04/99 (W-SN339220). (0155)

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