U. S. Environmental Protection Agency, Contracts Management Division (MD-33), Attn: ORDSC, Research Triangle Park, NC 27711

66 -- FLUORIMAGER 595 & PENTIUM WORKSTATION SOL AE0319 POC Pamela E. Smith, Purchasing Agent, 919/541-0635 The U.S. Environmental Protection Agency (EPA) intends to negotiate sole source procurement with Molecular Dynamics, Inc., 928 E. Arques Avenue, Sunnyvale, CA 94086 for the acquisition of a turnkey dual-line Fluorimaging System. This Fluorimaging system shall be delivered configured with a multi-purpose dual excitation laser (488 and 514 nm) induced fluorescence scanner allowing for sample components to readable directly from gels, membranes, TLC, or micro titre plates labeled with a wide variety of single and dual fluorescent labeling protocols, and have sensitivity to not less than 1.0 fmole fluorescein end-labeled DNA in polyacrylamide gel. Selectable bandpass filters (530nm, 570nm, 590nm, 610nm) must be included. Data acquisition electronics shall be calibrated automatically to ensure run-to-run repeatability. Scan time shall be less than 2 minutes for 20x25 cm scan area for single color at standard resolution and less than 30 minutes for dual-color scan at high resolution and high sensitivity. The system shall include software which controls scanning, multiple(up to 4)color separation, image display and tools for both quantitative data analysis and final reports that is compatible with EPA's existing software and equipment. Additionally, the Fluorimaging system shall have 5 orders of magnitude dynamic range, digital signal values storage in log format for greater intensity discrimination of low signal values, conversion utility for compatibility of images files with Macintosh or PC-based system desktop publishing programs, applications software for display and results analysis, and automated 1-D electrophoresis analysis software. The associated computer work station shall minimally consist of DELL Pentium II 400 MHz Mini Tower System, 64mB SDRAM, 8GM or larger UATA/EIDE hard disk drive, 30x or faster variable CD_ROM drive, AGP video adapter with 4MB or larger video RAM, PCI, SCSI interface card, and 3 COM Fast Etherlink 10/100 BASE-TX Network, 104-key keyboard, two button mouse, Windows NT 4.0 or higher, MS Office Pro, and image analysis and display software. EPA believes Molecular Dynamics, Inc., is the only vendor known of commercially available equipment which will interface directly with EPA's existing family of machines (PhosphorImager, Densitometer, and FluorSep). Any firm which feels it is capable of satisfying the requirements stated above should fully explain their capabilities in writing within 15 days of the date of this announcement. No Solicitation is available. All interested parties who believe they can provide the required supplies should fully explain their capabilities in writing within 15 days of the date of this announcement. The intent of this synopsis is to determine if any sources (s) exist and, therefore, no RFP is available. Consequently, any response(s) failing to provide the aforementioned data but instead choosing to merely submit a routine letter requesting a copy of the RFP will be ignored. Submit response to the U.S. EPA, `Attn: Pamela E. Smith (MD-33), Contracts ManagementDivision, Research Triangle Park, NC 27711. No telephonic or faxed request will be honored. Posted 06/07/99 (W-SN340157). (0158)

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