National Institute of Diabetes, Digestive & Kidney Diseases (NIDDK), Acquisition Management Branch, Natcher Building, 45 Center Dr., MSC 6600, Room 6AN32, Bethesda, Maryland 20892-6600

66 -- SELDI PROTEIN BIOLOGY SYSTEM II OR EQUIVALENT SYSTEM SOL NIDDK-RFQ 99-461 DUE 070699 POC Bryan Salas, 301-594-8855 The National Institutes of Health (NIH) National Institute of Diabetes and Digestive and Kidney Diseases (NIDDK) has a requirement to purchase one (1) SELDI Protein Biology System II with ProteinChip Reader with TLF upgrades, and SELDI Protocol Assistant Software or an equivalent system. The equipment shall be capable of the following unique performance specifications: 1) The system shall perform surface-enhanced affinity capture (SEAC) using surface-enhanced laser description/ionization (SELDI) for the quantitative analysis of trace components (at low femtomole levels) directly from biological samples. 2) The system shall perform analyses of protein chips comprising arrays of antibodies to capture diagnostic marker proteins directly from biological specimens for identification based on molecular mass. 3) The system shall perform the analysis of one microliter of unfractionated biological fluid as well as extract to provide a protein retentate map with a minimum of 300 features (peaks). 4) The system shall purify proteins from complex biological mixtures directly on the protein chip surface. 5) The system shall perform on-chip molecular processing, including protein purification, peptide mapping, C-terminal, microsequencing and post-translational modification analysis. 6) The system shall be capable of performing surface-enchanced neat desorption (SEND) for the analysis of low molecular mass analytes from 0-1,000 daltons. 7) The system shall provide experimental data in gel-view display format for direct comparison with gel electrophoresis data. 8) The system shall provide automated peak integration (digitization) with peak map display and comparison formats for differential protein display. 9) The system shall perform analyses of protein chip molecular arrays in a fully automated mode requiring a minimum of 100 addressable regions per 2mm of surface area. 10) The contractor shall provide a YEAR 2000 Warranty for each hardware, software, and firmware product delivered under this purchase order will be provided in the Request for Quote package. The standard industrial code (SIC) is 3826 and the business size is 500 employees. All responsible sources may submit a quotation, which if timely received, shall be considered by the agency. All responses shall be within the Simplified Acquisition Threshold for Commercial purchases up to $5 million. It is anticipated that Request for Quotation (RFQ) NIH-NIDDK-99-461 will be available fifteen (15) days after the publication date of this synopsis. Receipt of quotations will be due (10) ten days after the release of the RFQ. The anticipated award date is July 8, 1999. Requests for copies of the RFQ must be submitted in writing and may be FAXED to (301)480-4226. Quotations must be submitted in writing to the National Institute of Diabetes & Digestive & Kidney Diseases (NIDDK) Building 45, Room 6AN32, 45 Center Drive, MSC 6600, Bethesda, MD 20892-6600, Attention: Bryan Salas. FAX quotations must contain the company name, address, telephone number and FAX number. FAX quotations will only be accepted if dated and signed by an authorized company representative. Posted 06/09/99 (W-SN341177). (0160)

Loren Data Corp. http://www.ld.com (SYN# 0306 19990611\66-0004.SOL)