NCI Frederick Cancer Research and Development Center (NCI-FCRDC), P.O. Box B, Frederick, Maryland 21702-1201

A -- NIH INITIATIVE TO OBTAIN CLONES AND THE FULL LENGTH SEQUENCE FOR CDNA'S REPRESENTING EACH HUMAN GENE, AS WELL AS FOR GENES FROM THE MOUSE AND POSSIBLY OTHER MAMMALS. SOL S00-1 DUE 081399 POC Jane A. Wells, Sr. Contract Specialist, 301-846-5423, Contracting Officer-Dennis J. Dougherty, 301-846-1087 E-MAIL: Jane A. Wells, Sr. Contract Specialist, jwells@mail.ncifcrf.gov. Introduction: R&D -- Potential Sources Sought, SAIC,NIH,NCI, Introduction of procurement officials at SAIC, P O Box B, Frederick, MD 21702-1201. The National Institutes of Health (NIH) is initiating a new program to obtain clones and the full-length sequence for cDNA's representing each human gene, as well as for genes from the mouse and possibly other mammals. This will be a multi-Institute effort that will build a publicly accessible resource of sequences and clones, along with associated informatics tools, which is anticipated to be a key platform for biomedical research. Libraries that have a significant proportion of full-length or near full-length clones are being constructed, and it is anticipated that a large number of clones will be available for sequencing in the near future. One of the activities of the NIH Full-Length cDNA initiative is evaluation of the quality of currently available libraries, development and assessment of additional libraries enriched for full-length cDNA clones, and identification of a unique set of full-length cDNA clones. To accommodate the demand for sequencing these cDNA clones, it will be necessary to establish sequencing centers with demonstrated ability to sequence cDNA clones cost-effectively and at high throughput. The specific goal of this project is to determine the sequence of 5,000 clones the first year, and to develop the overall capacity to scale to 20,000 full-length mammalian cDNA's annually by 2001 and to make the sequence data publicly available in a timely manner. Organizations interested in participating in the establishment of high-throughput, efficient and cost-effective sequencing centers, with the intent of building the capability for rapidly sequencing the complete set of human, mouse and possibly other mammalian full-length cDNA's are requested to send or e-mail letters of interest to the addresses contained herein. Multiple multi-year awards are anticipated. Posted 06/23/99 (W-SN346335). (0174)

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