U. S. Environmental Protection Agency, Contracts Management Division (MD-33), Attn: ORDSC, Research Triangle Park, NC 27711

A -- DATA ON THE PHYTOTRANSFORMATION PROCESSES FOR THE DEGRADATION OF PCE SOL UKC019 DUE 072099 POC Pamela E. Smith, Purchasing Agent(919) 541-0635 THIS IS A COMBINED SYNOPSIS/SOLICITATION FOR COMMERCIAL ITEMS PREPARED IN ACCORDANCE WITH THE FORMAT IN SUBPART 12.6, AS SUPPLEMENTED WITH ADDITIONAL INFORMATION INCLUDED IN THIS NOTICE. THIS ANNOUNCEMENT CONSTITUTES THE ONLY SOLICITATION; QUOTES ARE BEING REQUESTED AND A WRITTEN SOLICITATION WILL NOT BE ISSUED. The solicitation number is UKC019 and the solicitation is being issued as a Request for Quote (RFQ). The solicitation document and incorporated provisions and clauses are those in effect through Federal Acquisition Circular 97-11. The Environmental Protection Agency (EPA) anticipates that the contract will be awarded as a result of full and open competition. A firm-fixed-price purchase order is anticipated to result from the award of this solicitation. The associated Standard Industrial Code (SIC) is 8731. This procurement requires the contractor to provide data on the phytotransformation processes for the degradation of the chlorinated solvent perchloroethene (PCE). The data are specific for the Orlando Naval Training Center, Orlando, FL. The samples and data are thus site specific. STATEMENT OF WORK: (1). Develop and provide general analytical methods, for investigating natural attenuation and the phytotransformation of PCE. Analytical methods needed to be developed to monitor the volatile compounds PCE, TCE, cis and trans-dichloroethene and vinyl chloride in sediment cores and in plant materials in the low ppb range. The contractor must insure that the chlorinated organic compounds do not volatilize from the samples. Also methods shall be developed to isolate and quantitatively analyze for derived chlorinated ethanols and haloacetic acids. Report shall include all raw data and be submitted on paper and computer disk. The contractor shall provide chromatogram, retention times and standards of all transformation products. Collect surface sediment and 8 core samples (from surface down to 40ft) from a PCE contaminated site at the former Orlando Naval Training School, Orlando, FL. (2). A contractor for the Orlando Naval Training Center will provided 10 sediment cores obtained from site. Characterize the 10 sediment/soil samples and aquifer material samples to include organic carbon, pH, Eh and size distribution. Determine sorption coefficients (Koc). Determine disappearance rate constants in triplicate (natural attenuation) for PCE in the 10 solids to water ratio of 1 to 5 (wight/weight). If TCE is formed as a major product, determine it's disappearance constant under the same reaction conditions in the solids samples in which it is detected. Transects showing the sampling location shall be provided. Product shall include all data, detailed write up of field sampling protocols, details of data attenuation and kinetic transformation rate constants. The report shall be submitted on paper and computer disk. (3). Collect 10 samples of representative terrestrial and wetland vegetation from the Orlando Naval Training School Site, Orlando, FL. EPA shall accompany the contractor to the site and assist in the plant selection. Split the samples for the following two sets of measurement. One part of each of the 10 plants shall be analyzed for PCE, TCE, dichloroethene, the derived chlorinated ethanols and chlorinated ethanols and chlorinatedf acetic acids. The other part of each of the 10 samples shall be incubated with PCE, TCE, dichloroethene, the derived chlorinated ethanols and chlorinated acetic acids. The other part of each of the 10 samples shall be incubated with PCE to test for enzymatic activity. This shall be done by determining disappearance rate constants for the plant samples using 100ppb of PCE dissolved in water and 1 to 5 ratio of plant to water ( wet weight/weight). Products should be identified when and where possible. Estimate contaminant mass balance and provide reaction pathways for the plant systems that show degradation of PCE. Report shall include all data, copies of methods and materials and computer files. The report shall be submitted on paper and computer disk. (4). Based on the results of the above studies, develop and analytical plan to monitor the PCE in sediments/soils and plants and its major degradation products at the Orlando Naval Training site. Provided the necessary kinetic and product distribution data for modeling and evaluation of phytoremediation. This plan shall include the number of plant samples and soil samples to be analyzed, the PCE products to analyze for, the time intervals for analysis. The report shall be submitted on paper and computer disk. PERIOD OF PERFORMANCE: Effective date of purchase order -- March 15, 2000. Quotations will be evaluated using the following technical capabilities criteria: 1.) Experience with similar upgrade projects; 2.) Past performance -- provide 3 references. Award will be made to the lowest priced offer meeting all of the technical requirements. The following FAR provisions apply to this solicitation: 52.212-1, Evaluation-Instructions to Offerors-Commercial; 52.212-2, Evaluation-Commercial Item. Evaluation criteria to be included in paragraph (a) of provision 52.212-2 are as follows: (I) technical capability to meet the Government requirements; (ii) past performance; (iii) price, in decreasing order of importance. Technical and past performance, when combined, are significantly more important than price. All offerors are to include with their offers a completed copy of provision 52.212-3, Offeror Representations and Certifications-Commercial Items. The following FAR clause applies to this acquisition: 52.212-4, Contract Terms and Conditions-Commercial Items and 52.217-7, Option for Increased Quantity -- Separately Priced Line Item. The following additional FAR clauses which are cited in clauses 52.212-5 are applicable to the acquisition: 52.203-6, Restrictions on Subcontractor Sales to the Government, with Alternate I; 52.203-10, Price or Fee Adjustment for Illegal or Improper Activity; 52.219-14, Limitation on Subcontracting; 52.222-26, Equal Opportunity, 52.222-35, Affirmative Action for Special Disabled and Vietnam Era Veterans; 52.222-36 Affirmative Action for Handicapped Workers; 52.222-37, Employment Reports on Special Disabled Veterans and Veterans of the Vietnam Era; 52.222-41, Service Contract Act of 1965, As Amended. Cost and technical information should be submitted in separate proposals. Please submit two copies of the technical proposal and two copies of the cost proposal to Pamela E. Smith, Purchasing Agent, U.S. Environmental Protection Agency, MD-33, Research Triangle Park, NC 27711. Courier Delivery Address is: U.S. Environmental Protection Agency, Receptionist-Administration Bldg. Lobby, Attn: Pamela E. Smith, 79 Alexander Drive, Research Triangle Park, NC 27709. All offers are due by July 20, 1999, 4:00 p.m. ET. No telephonic or faxed requests will be honored. References can be found at http://www.epa.gov/oam/rtp_cmd. Posted 07/06/99 (W-SN350789). (0187)

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