National Cancer Institute, Research Contracts Branch, PSAS, 6120 Executive Blvd, EPS/Room 638, Bethesda, MD 20892-7227

A -- VHL AND MET GENE TESTING SOL RFQ90151-NS DUE 081699 POC Patricia Haun, Purchasing Agent, 301-402-4509 The National Cancer Institute (NCI), Urologic Oncology Branch (UOB) intends to procure VHL and MET gene testing services and analysis on blood/tumor samples from the University of Pennsylvania, Genetic Diagnostic Referral Service (GDRS), Box 588, 340 Spruce Street, Philadelphia, PA 19184. The National Cancer Institute has a need for both tests. Mutations in the MET gene have been identified in papillary kidney cancer within the last 2 years and clinical testing is not generally available. In addition, NCI has a clinical need to get VHL gene testing performed on samples. The VHL gene, which was identified in 1993, is the gene for the hereditary form of renal carcinoma associated with the familial cancer syndrome, Von Hippel Lindau Disease. VHL is also the gene for the sporadic form of kidney cancer. Identification of mutations of the VHL gene in the germline of at-risk individuals from VHL families and in tumor tissue from patients with renal carcinoma is critical to the diagnosis of these diseases. Complete VHL gene mutation analysis is essential. Specifically, DNA shall be evaluated for rearrangement testing. Additionally, sequencing of all three exons shall occur if a mutation is not detected by rearrangement testing. GDRS of the University of Pennsylvania is the only source known to the NCI researcher that can provide complete VHL gene mutation analysis and MET gene testing. GDRS evaluates DNA for rearrangement testing and sequences all three exons if a mutation is not detected by rearrangement testing. This work must be performed in a CLIA approved laboratory, and all results must be reviewed by two board certified clinical molecular geneticists with results only being reported when both agree on the interpretation of the results. This notice is not a request for competitive quotation. However, if any interested party believes it can meet the above requirements it may submit a capability statement. Information furnished must be in writing and must contain material in sufficient detail to allow NCI to determine if the party can perform the requirement. Capability statements must be received in the contracting office by 3:00 p.m. EST, on August 16, 1999. A determination by the Government not to compete this proposed requirement based upon responses to this notice is solely within the discretion of the Government. Information received will be considered solely for the purpose of determining whether to conduct a competitive procurement. Posted 07/28/99 (W-SN360047). (0209)

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