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66 -- REQUIREMENTS CONTRACT FOR ONE TO FIVE DOUBLE FOCUSING MAGNETIC SECTOR MASS SPECTROMETERS SOL 1999C00253 DUE 081999 POC William Bancroft, Contract Specialist, (770) 488-2611 NOTE: The synopsis which was posted on July 30, 1999, is amended. Under Design Specifications, delete sections (g) through (m) and replace with the following revised text: (g) Analysis of TCDD in selected ion monitoring (SIM) mode at 10,000 resolution while selecting two masses from natural 2,3,7,8-TCDD (m/z 319.8965 and 321.8936) and masses from 13C12-2,3,7,8-TCDD (m/z 331.9368 and 333.9339) plus a lock mass and a lock mass check (m/z 316.9824) from PFK (the Dwell time on any one ion may not exceed 120 msec), will provide signal-to-noise ratios on raw data at m/z 321.8936 from 100 femtograms of a 2,3,7,8-TCDD standard (Cambridge Isotope Laboratory) injected on the top of a 30 meter DB-5 or DB-5MS GC column of: S/N greater than or equal to 125:1 where noise is peak to peak measurement encompassing 95% of noise data points (equivalent to four standard deviations, i.e., plus or minus 2 sigma about the mean). The measured isotope ratio between the 319.8965/321.8936 ions must be 0.77 +/- 10%. S/N greater than or equal to 250:1 where noise is mean positive-going noise to mean negative-going noise about the mean (EPA Protocol 8290; equivalent to two standard deviations, i.e., plus or minus 1 sigma about the mean). These values are based on a GC peak width at half height of two seconds. The cycle time for selecting all four masses plus the lock mass will be about 0.5 seconds. For other values of GC peak width, the signal-to-noise ratios may be calculated by assuming they are inversely proportional to the GC peak width. On smoothed data, when the GC peak width will be increased by no more than 20%, the signal-to-noise ratios may be increased by a factor of greater than 1.4, i.e.: S/N greater than or equal to 175:1 where noise is peak to peak measurement equivalent to four standard deviations. S/N greater than or equal to 350:1 where noise is peak to peak measurement equivalent to two standard deviations. One to five picograms of 13C12-2,3,7,8-TCDD will be co-injected with the 100 femtograms of 2,3,7,8-TCDD standard. (h) Analysis of TCDD in selected ion monitoring (SIM) mode at 10,000 resolution while selecting two masses from natural 2,3,7,8-TCDD (m/z 319.8965 and 321.8936) and masses from 13C12-2,3,7,8-TCDD (m/z 331.9368 and 333.9339) plus a lock mass check (m/z 316.9824) from PFK (the dwell time on any one ion may not be greater than 120 msec) will provide signal-to-noise ratios on raw data at m/z 321.8936 from 10 femtograms of a 2,3,7,8-TCDD standard injected on the top of a 30 meter DB-5 or DB-5MS GC column of: S/N greater than or equal to 12.5:1 where noise is peak to peak measurement encompassing 95% of noise data points (equivalent to four standard deviations, i.e., plus or minus 2 sigma about the mean). The measured isotope ratio between the 319.8965/321.8936 ions must be 0.77 +/- 10%. These values are based on a GC peak width at half height of two seconds. The cycle time for selecting all four masses plus the lock mass will be about 0.5 seconds. On smoothed data, when the GC peak width will be increased by nomore than 20%, the signal-to-noise ratios may be increased by a factor of greater than 1.4, i.e.: S/N greater than or equal to 17.5:1 where noise is peak to peak measurement equivalent to four standard deviations. One Hundred (100) to 500 femtograms of 13C12-2,3,7,8-TCDD will be co-injected with the 10 femtograms of 2,3,7,8-TCDD standard. (i) Analysis of 2,3,7,8-TCDD at a resolution greater than 20,000 RP (10% valley) must be demonstrated. (j) The mass spectrometer must demonstrate running 2,3,7,8-TCDD on a 30 or 60 meter DB-5 or DB-5MS showing chromatograms and calibration curves for 10 fg to 200 fg TCDD (Cambridge Isotope Laboratories standards) at 10,000 resolution. Ions monitored shall be 2 native TCDD (319.8965 and 321.8936), two ions for the 13C12-2,3,7,8-TCDD (m/z 331.9368 and 333.9339) and one ion for 13C6-2,3,7,8-TCDD (m/z 331.9078). The standard should contain various amounts of native TCDD from 10 fg to 200 fg, and an appropriate amount of 13C12-2,3,7,8-TCDD and 13C6-2,3,7,8-TCDD. (k) The dynamic range on the instrument must be demonstrated by consecutively injecting 2,3,7,8-TCDD samples from 5 fg to 500 pg monitoring in the SIM mode 2 native TCDD (319.8965 and 321.8936) and two ions for the 13C12-2,3,7,8-TCDD (m/z 331.9368 and 333.9339). (l) The GC-HR-SIM stability test specification. Initially a 5 point calibration curve covering a range from 0.25 pg/uL to 100 pg/uL of Cl4 to Cl8 dioxins, and furans will be created. The calibration standards concentrations correspond to one half that of CS1 to CS5 as defined in table 4 of the EPA 1613 protocol (Rev B). The standards will be injected in the splitless mode onto a 30 or 60 meter DB-5 or DB-5 MS column. The mass spectrometer will operate at 10,000 resolution throughout the test. All ions required by EPA 1613 (Rev B) will be monitored in 5 functions (groups) The %RSD of the relative response factors must be less than 20% for the five points and all isotope abundance must be within the acceptable limit (+/- 15%).. Over a 48 hour period the medium level standard (one half the concentration of CS3) should be run a minimum of four times. When compared to the relative response factor from the curve, the relative percent difference should not exceed 20% and all isotope abundance ratios must be within the acceptable limit (+/- 15%). (m) Chromatograms for all the analysis including the TCDD analysis and the multi group analysis showing signal-to-noise data must be included in the bid to show quality of data. All other terms and conditions remain unchanged. Posted 08/05/99 (I-SN364129). (0217)

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