National Institutes of Health, National Heart, Lung, and Blood Institute, Contracts Operations Branch, 6701 Rockledge Drive, Room 6100, MSC 7902, Bethesda, MD 20892

A -- RESEARCH & DEVELOPMENT SOL NHLBI-PS-99-482 DUE 082199 POC Cecilia D. Morales, Purchasing Agent, (301)435-0369, Debra C. Hawkins, Chief, Procurement Section, (301)435-0366 The National Heart, Lung, and Blood Institute intends to negotiate on a sole source basis with Dr. William G. Kearns, 1416 S. Veaux Loop, Norfolk, VA 23509, to conduct Research of specialized interphase FISH, Fiber-FISH, 24-color FISH techniques and comparative genomic hybridization on cells from patients with myelodysplastic syndrome and aplastic anemia. In order to continue and maintain the role of cytogenetic analysis in the diagnosis and treatment of hematological disorders study, and due to the knowledge and specialization of Dr. William G. Kearns on the development of FISH, an award to any other source for this study would result in a substantial expense to the Government that is not expected to be recovered through competition. Dr. Kearns collaborated with the Hematology Branch in July 1998 in an effort to decide whether FISH can detect additional chromosome abnormalities not found by high resolution G-band karyotyping in bone marrow cells from patients with aplastic anemia and myelodysplastic syndrome. Multicolor interphase FISH was performed on non-dividing, interphase nuclei. Over 400 cells were analyzed per patient, over 65 patients were enrolled in the study. In this study, Dr. Kearns will use the following: DNA clones to identify structural and numerical chromosome abnormalities, comparative genomic hybridization to identify chromosome regions and DNA-RNA FISH to study gene expresion. The specialized FISH techniques will identify chromosome abnormalities, characterize marker chromosomes, monitor effects of therapy, detect residual disease, determine the origin of marrow cells following bone marrow transplantation, identify chromosome regions of amplification or deletion, detect chromosome abnormalities in non dividing cells, and determine the lineage of cytogenetically abnormal cell clones. PLACE OF PERFORMANCE: Work will be performed at Building 10, Room 7C114, 9000 Rockville Pike, Bethesda, MD 20892. PERIOD OF PERFORMANCE: The period of performance is twelve (12) months. This acquisition is being conducted under simplified acquisition procedures and is exempt from the requirements of FAR Part 6. The Standard Industrial Classification (SIC) Code is 8731, Size Standard 500. This notice of intent is not a request for competitive proposals. Interested parties may identify their interest and capabilities in response to this requirement. The determination by the Government not to compete the proposed contract based upon responses to this notice is solely within the discretion of the Government. Information received will normally be considered solely for the purpose of determining whether to conduct future competitive procurement. Responses to this announcement, referencing synopsis number NHLBI-PS-99-482 may be submitted to the National Heart, Lung, and Blood Institute, Contracts Operations Branch, Procurement Section, Building RKL2, Room 6149, 6701 Rockledge Drive, Bethesda, MD 20892-7902, Attention Cecilia D. Morales, Purchaisng Agent. All responsible sources may submit a quotation, which if timely received, shall be considered by the agency. Posted 08/05/99 (W-SN363720). (0217)

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